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Team:The University of Alberta/6 August 2008
From 2008.igem.org
(Difference between revisions)
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| + | '''Chris''' | ||
| + | *PCR'd Tryp out of pUC57 (the original that Mike supplied)...buuut I used Vf and Vr to do it. I shouldnt have gotten any results because pUC57 doesnt have sites for those primers to bind BUT I did get a band about the right size...nevertheless, whatever was amplified wouldnt have the biobrick suffix and prefix in it so it was discarded. | ||
| + | *Redid the PCR with the correct primers....got a good, sharp band ~1500bp in size BUT the same band was in the negative control. I didnt want to risk using something that was just a random 1500bp fragment so.. | ||
| + | *Gel extracted the 1500bp fragment from the PCR sample | ||
| + | *I began sequencing it (Sequencing was finished by David and Kelly). Will take samples to MBSU tomorrow | ||
| + | |||
'''Saima''' | '''Saima''' | ||
*Mini-preped the O/N's of Col. 4,5,& 6 of I0500+ER01 and I0500+ER02. Can be found in Mini-prep Box 3. | *Mini-preped the O/N's of Col. 4,5,& 6 of I0500+ER01 and I0500+ER02. Can be found in Mini-prep Box 3. | ||
*Sequenced Minipreps of Col.4 & Col.5 of I0500+ER01, and Col.4 of I0500+ER02. To be taken to MBSU on Thrursday. Results to be back by Friday. | *Sequenced Minipreps of Col.4 & Col.5 of I0500+ER01, and Col.4 of I0500+ER02. To be taken to MBSU on Thrursday. Results to be back by Friday. | ||
*Set up the O/N's for I0500+ER01 col.6, I0500+ER02 col.4, I0500 in new vector, GFP+B0034 Col.1a & 1b. | *Set up the O/N's for I0500+ER01 col.6, I0500+ER02 col.4, I0500 in new vector, GFP+B0034 Col.1a & 1b. | ||
Revision as of 16:35, 8 August 2008
Chris
- PCR'd Tryp out of pUC57 (the original that Mike supplied)...buuut I used Vf and Vr to do it. I shouldnt have gotten any results because pUC57 doesnt have sites for those primers to bind BUT I did get a band about the right size...nevertheless, whatever was amplified wouldnt have the biobrick suffix and prefix in it so it was discarded.
- Redid the PCR with the correct primers....got a good, sharp band ~1500bp in size BUT the same band was in the negative control. I didnt want to risk using something that was just a random 1500bp fragment so..
- Gel extracted the 1500bp fragment from the PCR sample
- I began sequencing it (Sequencing was finished by David and Kelly). Will take samples to MBSU tomorrow
Saima
- Mini-preped the O/N's of Col. 4,5,& 6 of I0500+ER01 and I0500+ER02. Can be found in Mini-prep Box 3.
- Sequenced Minipreps of Col.4 & Col.5 of I0500+ER01, and Col.4 of I0500+ER02. To be taken to MBSU on Thrursday. Results to be back by Friday.
- Set up the O/N's for I0500+ER01 col.6, I0500+ER02 col.4, I0500 in new vector, GFP+B0034 Col.1a & 1b.
