Wisconsin: Lignin Project/27 June 2008
From 2008.igem.org
(Difference between revisions)
Line 9: | Line 9: | ||
They were verified to work on a gel and extracted using a Qiagen Gel Extraction Kit.<br> | They were verified to work on a gel and extracted using a Qiagen Gel Extraction Kit.<br> | ||
Unfortunately not enough DNA was obtained to perform a ligation.<br> | Unfortunately not enough DNA was obtained to perform a ligation.<br> | ||
+ | Overnight culture started using revised C-Media recipe: growth after overnight incubation at 30C was observed<br> | ||
+ | Single colony overnight culture started for growth curve following morning.<br> | ||
'''Team Fungus:''' <br> | '''Team Fungus:''' <br> | ||
Plate with 1:3 insert to vector ratio had 2 colonies<br> | Plate with 1:3 insert to vector ratio had 2 colonies<br> |
Latest revision as of 19:49, 18 October 2008
Team Sorbitol:
Checked the PCR product from last night on a gel and found several bands to work again (700bp). |