Wisconsin: Lignin Project/1 July 2008

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This time we obtained enough insert to do a ligation but not enough vector.<br>
This time we obtained enough insert to do a ligation but not enough vector.<br>
To solve this we grew 40mL of DH5a with pBAD30 overnight at 30C<br>
To solve this we grew 40mL of DH5a with pBAD30 overnight at 30C<br>
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'''Team Fungus:'''<br>
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Ran yet another PCR. Running low on cDNA.<br>

Revision as of 16:23, 11 August 2008

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Team Sorbitol:

The overnight PCR reaction failed.
Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before.
The digestion was verified on a gel and extracted using the Qiagen kit.
This time we obtained enough insert to do a ligation but not enough vector.
To solve this we grew 40mL of DH5a with pBAD30 overnight at 30C
Team Fungus:

Ran yet another PCR. Running low on cDNA.
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