Team:KULeuven/Freezer

From 2008.igem.org

(Difference between revisions)
(→Parts stored in the fridge in the lab (4°C))
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|- style="background: #E0FFFF; text-align: center;"
|- style="background: #E0FFFF; text-align: center;"
|dNTP mix||ATP, GTP, TTP, CTP||90||2 mM||28||August 07  
|dNTP mix||ATP, GTP, TTP, CTP||90||2 mM||28||August 07  
 +
|}
 +
 +
 +
==Ligations: step 1==
 +
{|
 +
|- style="background: #003E81;color:white;width=100%"
 +
! colspan="6" style="background: #003E81" | Inputs
 +
|- style="background: #99CCFF; text-align: center; width=100%"
 +
|width=200| '''Name'''
 +
|width=160| '''Miniprep'''
 +
|width=150| '''Digest'''
 +
|width=150| '''Ligation'''
 +
|width=150| '''Colonies'''
 +
|width=150| '''Confirmed'''
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|J23116+B0032||-||-||-||-||-
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|C0040+B0015||ok||ok||-||-||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|R0040+E0240||-||-||-||-||-
 +
|- style="background: #003E81;color:white;width=100%"
 +
! colspan="6" style="background: #003E81" | Output
 +
|- style="background: #99CCFF; text-align: center; width=100%"
 +
|width=200| '''Name'''
 +
|width=160| '''Miniprep'''
 +
|width=150| '''Digest'''
 +
|width=150| '''Ligation'''
 +
|width=150| '''Colonies'''
 +
|width=150| '''Confirmed'''
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| R0084+B0032||ok||ok||ok||-||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| E0022+B0015||ok||ok||ok||ok||ok
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| K145015+B0015||pcr||ok||ok||ok||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| E0040+B0015||-||-||-||-||-
 +
|- style="background: #003E81;color:white;width=100%"
 +
! colspan="6" style="background: #003E81" | Memory
 +
|- style="background: #99CCFF; text-align: center; width=100%"
 +
|width=200| '''Name'''
 +
|width=160| '''Miniprep'''
 +
|width=150| '''Digest'''
 +
|width=150| '''Ligation'''
 +
|width=150| '''Colonies'''
 +
|width=150| '''Confirmed'''
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| ? ||-||-||-||-||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| ? ||-||-||-||-||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| ? ||-||-||-||-||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| ? ||-||-||-||-||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| ? ||-||-||-||-||-
 +
|- style="background: #003E81;color:white;width=100%"
 +
! colspan="6" style="background: #003E81" | Filter
 +
|- style="background: #99CCFF; text-align: center; width=100%"
 +
|width=200| '''Name'''
 +
|width=160| '''Miniprep'''
 +
|width=150| '''Digest'''
 +
|width=150| '''Ligation'''
 +
|width=150| '''Colonies'''
 +
|width=150| '''Confirmed'''
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| R0084+J23022 ||ok||ok||ok||-||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| J230109+J23032 ||ok||ok||ok||ok||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| K145014+B0015 ||-||-||-||-||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| K145001+B0015 ||-||-||-||-||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| I7122074+J23032 ||ok||ok||ok||ok||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| IK0145015+B0015 ||pcr||ok||ok||ok||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| E0040+B0015 ||-||-||-||-||-
 +
|- style="background: #003E81;color:white;width=100%"
 +
! colspan="6" style="background: #003E81" | Inverter
 +
|- style="background: #99CCFF; text-align: center; width=100%"
 +
|width=200| '''Name'''
 +
|width=160| '''Miniprep'''
 +
|width=150| '''Digest'''
 +
|width=150| '''Ligation'''
 +
|width=150| '''Colonies'''
 +
|width=150| '''Confirmed'''
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| I712074+J23032||ok||ok||ok||ok||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| C0012+B0015||ok||ok||ok||-||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| R0011+F1610||ok||-||-||-||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| R0011+E0240||-||-||-||-||-
 +
|- style="background: #003E81;color:white;width=100%"
 +
! colspan="6" style="background: #003E81" | Reset
 +
|- style="background: #99CCFF; text-align: center; width=100%"
 +
|width=200| '''Name'''
 +
|width=160| '''Miniprep'''
 +
|width=150| '''Digest'''
 +
|width=150| '''Ligation'''
 +
|width=150| '''Colonies'''
 +
|width=150| '''Confirmed'''
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| I712074+J023022||ok||ok||ok||ok||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| C0012+B0015||-||ok||ok||ok||-
 +
|- style="background: #003E81;color:white;width=100%"
 +
! colspan="6" style="background: #003E81" | Cell Death
 +
|- style="background: #99CCFF; text-align: center; width=100%"
 +
|width=200| '''Name'''
 +
|width=160| '''Miniprep'''
 +
|width=150| '''Digest'''
 +
|width=150| '''Ligation'''
 +
|width=150| '''Colonies'''
 +
|width=150| '''Confirmed'''
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| J23100+B0032||ok||ok||ok||ok||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| C0062+B0015||-||ok||ok||ok||ok
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| R0062+B0032||ok||ok||ok||-||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| P1010+B0015||-||ok||ok||ok||-
 +
|- style="background: #E0FFFF; text-align: center;"
 +
| R0062+E0240||-||-||-||-||-
|}
|}

Revision as of 13:14, 11 August 2008

  dock/undock dropdown  


Parts stored in the fridge in the lab (4°C)

Plasmids: MiniPrep Products
Part Plasmid Description µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_M30109 M30109]pSB1AC3Light-sensor+/- 4050.41July 23
[http://partsregistry.org/Part:BBa_M30109 M30109]pSB1AC3Light-sensor+/- 3054.32July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]BBa_J61002 (Ap)Constitutive promoter+/- 60345.03 July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]BBa_J61002 (Ap)Constitutive promoter+/- 30176.14 July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 2073.85July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 3095.16July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 40104.07July 29
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 25177.18July 24
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 30161.79July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 4063.010July 23
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 10112.311July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 20130.312July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 45303.113July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 30213.614July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 48311.031July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 40214.047July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 40209.548July 29
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 4032.015July 23
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 3060.916July 24
[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2RBS (efficiency 0.3)+/- 10114.818July 24
[http://partsregistry.org/Part:BBa_E0022 E0022]pSB1A2eCFP+/- 4084.219July 23
[http://partsregistry.org/Part:BBa_E0022 E0022]pSB1A2eCFP+/- 4086.420July 24
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI+/- 30133.121July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI repressor+/- 40146.449July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI repressor+/- 40203.150July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 35117.423July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 39.5144.824July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 48129.128July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)3d key+ terminator+/- 40160.725July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)3d key+ terminator+/- 46308.329July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 46375.126July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 4840430July 29
[http://partsregistry.org/Part:BBa_I712074 I712074]BBa_J52017 (Ap,Km)T7 Promotor+/- 42.5208.227July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 30104.022July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 35170.845July 29
[http://partsregistry.org/Part:BBa_I712022 I712022]BBa_J52017 (Km, Ap)T7 polymerase+/- 40394.135July 31
[http://partsregistry.org/Part:BBa_R0062 R0062]pSB1A2LuxR regulated promoter +/- 40104.137July 31
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2LacI regulated promoter+/- 4564.043July 29
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2LacI regulated promoter +/- 4069.844July 29
[http://partsregistry.org/Part:BBa_I12006 I12006]pSB2K3434 cI repressible promoter +/- 4825.851August 08
[http://partsregistry.org/Part:BBa_I12006 I12006]pSB2K3434 cI repressible promoter +/- 4852.752August 08
[http://partsregistry.org/Part:BBa_J23116 J23116]BBa_J61002 (Ap)Constitutive promoter +/- 48328.853August 08
[http://partsregistry.org/Part:BBa_C0060 C0060]pSB1A2lactonase production+/- 4884.260August 11
[http://partsregistry.org/Part:BBa_C0062 C0062]pSB1A2LuxR repressor+/- 4876.261August 11
[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2RBS(medium)+/- 4851.062August 11
[http://partsregistry.org/Part:BBa_R0040 R0040]pSB1A2tetR repressible promoter+/- 4837.963August 11
[http://partsregistry.org/Part:BBa_P1010 P1010]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Cell Death+/- 48119.138August 06
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for TetR generator+/- 48131.039August 06
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for TetR generator+/- 48133.240August 06
[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Reset+/- 48118.541August 06
[http://partsregistry.org/Part:BBa_J23100 J23100]+[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2Ligation for Cell Death+/- 4641.042August 06
[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Cell Death+/- 46124.854August 08
[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]pSB1A2GFP with LVA tag+/- 46152.755August 08
[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1A2Ligation for Output+/- 4617.556August 08
[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)Ligation for Filter+/- 4689.957August 08
[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)Ligation for Filter+/- 4694.458August 08
[http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Output+/- 46151.859August 08


Restriction Digests
Part Enzyme 1 Enzyme 2 vector/insert length (bp) µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_J23100 J23100]EcoR ISpe IInsert5238.12.61July 24
[http://partsregistry.org/Part:BBa_C0040 C0040] EcoR ISpe IInsert677257.12July 24
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert12533.43.83July 24
[http://partsregistry.org/Part:BBa_E0022 E0022] EcoR ISpe IInsert779229.54July 24
[http://partsregistry.org/Part:BBa_J23109 J23109] EcoR ISpe IInsert52214.25July 29
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert125256.16July 29
[http://partsregistry.org/Part:BBa_I712074 I712074] EcoR ISpe IInsert62233.47July 29
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3090212.98July 30
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3090451.39July 31
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3308255.122August 07
[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector33033111.711July 31
[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector33032113.420August 07
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72208.512July 30
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72207.513July 30
[http://partsregistry.org/Part:BBa_C0062 C0062] EcoR ISpe IInsert7741824.714August 01
[http://partsregistry.org/Part:BBa_C0060 C0060] EcoR ISpe IInsert806158.215August 01
[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2] EcoR ISpe IPlasmid20791718.216August 01
[http://partsregistry.org/Part:BBa_P1010 P1010] EcoR ISpe IInsert682104.917August 01
[http://partsregistry.org/Part:BBa_K145015 K145015] (GFP-LVA) EcoR ISpe IInsert625136.318August 05
[http://partsregistry.org/Part:BBa_C0012 C0012] EcoR ISpe IInsert1145125.619August 05
[http://partsregistry.org/Part:BBa_J23022 J23022] EcoR IXba IVector34982015.221August 07
[http://partsregistry.org/Part:BBa_R0062 R0062] EcoR ISpe IInsert55277.823August 07
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3305+/-255.122August 07

Things stored in freezer in lab (-20°C)

Miscellaneous
Description Name µl left Concentration Box Date
Primer GFP-LVA10849020 µM1August 01
Primer GFP-LVA10859020 µM2August 01
dNTP mixATP, GTP, TTP, CTP7010 mM3August 01
Primer GFP-LVA1084500100 µM4August 01
Primer GFP-LVA1085500100 µM5August 01
Primer Test1074800100 µM6August 07
Primer Test1075780100 µM7August 07
Primer T7-UmuD1076560100 µM8August 07
Primer T7-UmuD1077477100 µM9August 07
Primer T7-UmuD1078467100 µM10August 07
Primer T7-UmuD107945100 µM11August 07
Primer Transduction1097627100 µM12August 07
Primer Transduction1098710100 µM13August 07
Primer Transduction1099608100 µM14August 07
Primer Anti-sense LuxI1100522100 µM15August 07
Primer Anti-sense LuxI1101237100 µM16August 07
Primer Test107410020 µM17August 07
Primer Test107510020 µM18August 07
Primer T7-UmuD107610020 µM19August 07
Primer T7-UmuD107710020 µM20August 07
Primer T7-UmuD107810020 µM21August 07
Primer T7-UmuD107910020 µM22August 07
Primer Transduction109710020 µM23August 07
Primer Transduction109810020 µM24August 07
Primer Transduction109910020 µM25August 07
Primer Anti-sense LuxI110010020 µM26August 07
Primer Anti-sense LuxI110110020 µM27August 07
dNTP mixATP, GTP, TTP, CTP902 mM28August 07


Ligations: step 1

Inputs
Name Miniprep Digest Ligation Colonies Confirmed
J23116+B0032-----
C0040+B0015okok---
R0040+E0240-----
Output
Name Miniprep Digest Ligation Colonies Confirmed
R0084+B0032okokok--
E0022+B0015okokokokok
K145015+B0015pcrokokok-
E0040+B0015-----
Memory
Name Miniprep Digest Ligation Colonies Confirmed
 ? -----
 ? -----
 ? -----
 ? -----
 ? -----
Filter
Name Miniprep Digest Ligation Colonies Confirmed
R0084+J23022 okokok--
J230109+J23032 okokokok-
K145014+B0015 -----
K145001+B0015 -----
I7122074+J23032 okokokok-
IK0145015+B0015 pcrokokok-
E0040+B0015 -----
Inverter
Name Miniprep Digest Ligation Colonies Confirmed
I712074+J23032okokokok-
C0012+B0015okokok--
R0011+F1610ok----
R0011+E0240-----
Reset
Name Miniprep Digest Ligation Colonies Confirmed
I712074+J023022okokokok-
C0012+B0015-okokok-
Cell Death
Name Miniprep Digest Ligation Colonies Confirmed
J23100+B0032okokokok-
C0062+B0015-okokokok
R0062+B0032okokok--
P1010+B0015-okokok-
R0062+E0240-----
Retrieved from "http://2008.igem.org/Team:KULeuven/Freezer"