Wisconsin: Lignin Project/31 July 2008

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|'''Team Sorbitol:'''<br>
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Competent cells for MG1655, pBAD30,pBAD18, and DH5a<br>
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<br>
|'''Team Fungus:'''<br>
|'''Team Fungus:'''<br>
Ran digestion of pET28a and ran out on .75% agrose gel.  All bands were correct size. <br>
Ran digestion of pET28a and ran out on .75% agrose gel.  All bands were correct size. <br>

Revision as of 21:05, 18 October 2008

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Team Sorbitol:

Competent cells for MG1655, pBAD30,pBAD18, and DH5a

Team Fungus:

Ran digestion of pET28a and ran out on .75% agrose gel. All bands were correct size.
Used the QIAquick gel extraction kit to purify the double digested vector band. Spec reading of 14 ng/uL.
Ran overnight ligation at 4 degrees C.

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