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EPF-Lausanne/11 August 2008
From 2008.igem.org
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=Molecular Biology= | =Molecular Biology= | ||
We try by different way to assemble biobrick | We try by different way to assemble biobrick | ||
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| + | We(Vincent) do a mistake | ||
==E1010 and F1610== | ==E1010 and F1610== | ||
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We successful have isolate from Gel : R0040, R0071 and B0034 | We successful have isolate from Gel : R0040, R0071 and B0034 | ||
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| + | We try to change strategy of isolate I1466 and I14033 | ||
| + | |||
| + | ==I1466/I14033== | ||
| + | I14033 is small. We let it in the vector. I1466 is big we use it to a insert. | ||
| + | We cut with SpeI and PstI. | ||
| + | We digest the plasmid and we are going to do tomorrow ligation. | ||
| + | |||
| + | |||
| + | ==Without gel, use PCR with biobrick primer== | ||
| + | We want | ||
| + | |||
http://openwetware.org/wiki/Knight:Annealing_and_primer_extension_with_Klenow_polymerase | http://openwetware.org/wiki/Knight:Annealing_and_primer_extension_with_Klenow_polymerase | ||
Revision as of 12:13, 12 August 2008
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Contents |
Molecular Biology
We try by different way to assemble biobrick
We(Vincent) do a mistake
E1010 and F1610
We try last week to assemble two big parts because it is easy to purify it. We do ligation and transformation last week. The resultat was great all of the ten plates work. Monday, we decide to do a mini prep of these cells to purifiy DNA. Then we want to check the plasmide so we digest a small quantity of them with EcoRI and SpeI to prepare it to do a gel tomorrow.
by Gel
We try again to purifiy insert and vector by Gel.
We try to assemble :
R0040/B0034
R0071/B0034
and I1466/I1433
We successful have isolate from Gel : R0040, R0071 and B0034
We try to change strategy of isolate I1466 and I14033
I1466/I14033
I14033 is small. We let it in the vector. I1466 is big we use it to a insert. We cut with SpeI and PstI. We digest the plasmid and we are going to do tomorrow ligation.
Without gel, use PCR with biobrick primer
We want
http://openwetware.org/wiki/Knight:Annealing_and_primer_extension_with_Klenow_polymerase
