"
Team:Paris/Modeling/f2
From 2008.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
[[Image:f2.png]] | [[Image:f2.png]] | ||
| - | Exactly as for the binding of IPTG on LacR (see [[Team:Paris/Modeling/estimation#how_to_control_the_concentration_of_the_transcription_factor_.3F|TF concentration control]]), leading to a lowered inhibition of ''Plac'', aTc binds to TetR. So, after having determinated the Hill function [expr.''Ptet''] = ƒ2(TetR) = ƒ2aux(IPTG), we can study [expr.''Ptet''] = ƒ2ter([TetR],[aTc]), and determine by calcul [TetR<sub> | + | Exactly as for the binding of IPTG on LacR (see [[Team:Paris/Modeling/estimation#how_to_control_the_concentration_of_the_transcription_factor_.3F|TF concentration control]]), leading to a lowered inhibition of ''Plac'', aTc binds to TetR. So, after having determinated the Hill function [expr.''Ptet''] = ƒ2(TetR) = ƒ2aux(IPTG), we can study [expr.''Ptet''] = ƒ2ter([TetR],[aTc]), and determine by calcul [TetR<sub>free</sub>] = ƒ2bis(aTc) = f2<sup>-1</sup>([expr.''Ptet'']), to endly give an analytical expression of ƒ2ter = ƒ2οƒ2bis |
Revision as of 18:52, 12 August 2008
No particular strain.
Exactly as for the binding of IPTG on LacR (see TF concentration control), leading to a lowered inhibition of Plac, aTc binds to TetR. So, after having determinated the Hill function [expr.Ptet] = ƒ2(TetR) = ƒ2aux(IPTG), we can study [expr.Ptet] = ƒ2ter([TetR],[aTc]), and determine by calcul [TetRfree] = ƒ2bis(aTc) = f2-1([expr.Ptet]), to endly give an analytical expression of ƒ2ter = ƒ2οƒ2bis
Otherwise, if we find in the litterature the dissociation constant of the reaction aTc + TetR⇄ aTc_TetR, we could determine by calculus the amount of free TetR, function of the expression of a known constitutive promoter before tetR and of the added aTc. In this way, we just have to study [expr.Ptet] = ƒ2(TetRfree).
