Team:Paris/Modeling/f2

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Revision as of 15:07, 13 August 2008

No particular strain.

To deduce from our measurements the correct hill function, see the different methods. The best one consist in finding in the literature the dissociation constant of the reaction aTc + TetR⇄ aTc_TetR, in order to determine by calculus the amount of free TetR, function of the expression of a known constitutive promoter before tetR and of the added aTc. In this way, we just have to study [expr.Ptet] = ƒ2(TetR_free).

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 No particular strain.
-[[Image:f2a.png]]
+[[Image:f2alt.png]]
-Exactly as for the binding of IPTG on LacR (see [[Team:Paris/Modeling/estimation#how_to_control_the_concentration_of_the_transcription_factor_.3F|TF concentration control]]), leading to a lowered inhibition of ''Plac'', aTc binds to TetR. So, after having determinated the Hill function [expr.''Ptet''] = &#131;2(TetR) = &#131;2aux(IPTG), we can study [expr.''Ptet''] = &#131;2ter([TetR],[aTc]), and determine by calcul [TetR<sub>free</sub>] = &#131;2bis(aTc) = f2<sup>-1</sup>([expr.''Ptet'']), to endly give an analytical expression of &#131;2ter = &#131;2&#959;&#131;2bis
+To deduce from our measurements the correct hill function, see the [[Team:Paris/Modeling/estimation#how_to_control_the_concentration_of_the_transcription_factor_.3F|different methods]].
+The best one consist in finding in the literature the dissociation constant of the reaction aTc + TetR&#8644; aTc_TetR, in order to determine by calculus the amount of free TetR, function of the expression of a known constitutive promoter before ''tetR'' and of the added aTc. In this way, we just have to study [expr.''Ptet''] = &#131;2(TetR<sub>free</sub>).
-Otherwise, if we find in the litterature the dissociation constant of the reaction aTc + TetR&#8644; aTc_TetR, we could determine by calculus the amount of free TetR, function of the expression of a known constitutive promoter before ''tetR'' and of the added aTc. In this way, we just have to study [expr.''Ptet''] = &#131;2(TetR<sub>free</sub>).
-[[Image:f2alt.png]]