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EPF-Lausanne/14 August 2008
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=Molecular biology= | =Molecular biology= | ||
| - | We do mini-prep of E1010, "F1610" and P0140 | + | We do a mini-prep of E1010, "F1610" and P0140 |
==E1010/F1610== | ==E1010/F1610== | ||
| - | Yesterday we | + | Yesterday we found out that F1610 has a size of 300bp, instead of 800bp. So there are two hypotheses: 1) The mini-prep is bad or 2) the biobrick from IGEM is bad. |
So we do another digestion serie with EcoRI and SpeI. We do with different mini-prep of F1610 and the last mini-prep of E1010 that work(for positiv control). | So we do another digestion serie with EcoRI and SpeI. We do with different mini-prep of F1610 and the last mini-prep of E1010 that work(for positiv control). | ||
Revision as of 09:18, 19 August 2008
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Molecular biology
We do a mini-prep of E1010, "F1610" and P0140
E1010/F1610
Yesterday we found out that F1610 has a size of 300bp, instead of 800bp. So there are two hypotheses: 1) The mini-prep is bad or 2) the biobrick from IGEM is bad.
So we do another digestion serie with EcoRI and SpeI. We do with different mini-prep of F1610 and the last mini-prep of E1010 that work(for positiv control).
Some mini-prep from F1610 are too small, there isn't enough liquid so we do PCR with these (we use E1010 from last mini-prep as a positiv control).
We do in same time PCR of all fragments that ligated E1010/F1610 to see if there is a fragment of 1500 bp.
Resultat of Gel
All mini-preps of F1610 have a fragment of 300 bp. So the biobrick from IGEM doesn't work. All ligated fragments E1010/F1610 aren't ligate.
(see Gel picture on page 75 in notebook of lab)
Test of specific primer of BBs
We do a PCR to see if specific primers that Alexandre commanded work with biobrick. We do gel tomorrow.
