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Alberta NINT/13 August 2008
From 2008.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
Sequenced TA2, TA3, TA6 and TA7. | Sequenced TA2, TA3, TA6 and TA7. | ||
Made overnights of the three colonies found on the pSB1A3 plate. | Made overnights of the three colonies found on the pSB1A3 plate. | ||
| + | |||
| + | lab work (JD): | ||
| + | From ligations: | ||
| + | TA2In=76 colonies | ||
| + | TA3In=90 colonies | ||
| + | TA6In=66 colonies | ||
| + | TA7In=68 colonies | ||
| + | Negative=4 colonies (from Wayne) | ||
| + | Created 4 O/N from each plate | ||
| + | Annealed TA2, TA3, TA6, TA7 | ||
| + | Nucleotide removal kit for TA2 TA3 TA6 TA7 | ||
| + | Restriction Digest of TA2, 3, 6, 7 with BamH1 and NcoI | ||
| + | Ran on gel 2% at 150V for 2 hours | ||
| + | [[Image:UVP00337.jpg]] | ||
[[Alberta_NINT/14_August_2008 | Next entry >]] | [[Alberta_NINT/14_August_2008 | Next entry >]] | ||
Revision as of 17:13, 15 August 2008
lab work (SD):
Isolated DNA of TA2, TA3, TA6 and Ta7 from overnights using QIAquick spin miniprep standard
protocol.
Sequenced TA2, TA3, TA6 and TA7.
Made overnights of the three colonies found on the pSB1A3 plate.
lab work (JD):
From ligations:
TA2In=76 colonies
TA3In=90 colonies
TA6In=66 colonies
TA7In=68 colonies
Negative=4 colonies (from Wayne)
Created 4 O/N from each plate
Annealed TA2, TA3, TA6, TA7
Nucleotide removal kit for TA2 TA3 TA6 TA7
Restriction Digest of TA2, 3, 6, 7 with BamH1 and NcoI
Ran on gel 2% at 150V for 2 hours
