User:University of Washington/13 August 2008

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Revision as of 22:04, 14 August 2008

Conjugation (Scott)

experiment from Aug. 7 results

experiment #	Bacteria	Yeast	Yeast count	transconjugation efficiency
1	250uL	250uL	66	2.64E-5
2	200uL	300uL	64	2.13E-5
3	100uL	400uL	32	8.00e-6
4	50uL	450uL	18	4.00e-6

LuxR from AraC and TetR(Faifan)

QuikChange Approach

- Overnight QuikChange#4 culture from Amp plate (reaction1 x 4, reaction2 x 2) in Tsy+Amp

- Sequence of DNA from QuikChange#3 came back ==> No mutation.

Elowitz's

-Restriction Digest of BBa_Elowitz, BBa_E0240(GFP), BBa_I0462(LuxR), BBa_P1010(on pSB3K3)

	dH2O(ul)	Buffer(ul)	BSA(ul)	DNA(ul)	E(ul)	X(ul)	S(ul)	P(ul)
BBa_Elowitz	32.5	EcoRI, 5	0.5	10	1	-	1	-
LuxR	32.5	NEB3, 5	0.5	10	-	1	-	1
GFP	32.5	NEB3, 5	0.5	10	-	1	-	1
P1010	32.5	EcoRI, 5	0.5	10	1	-	-	1

Back to Team:University_of_Washington/Notebook#Notebook

@@ Line 21: / Line 21: @@
 -Restriction Digest of BBa_Elowitz, BBa_E0240(GFP), BBa_I0462(LuxR), BBa_P1010(on pSB3K3)
-<table border=1 align="center">
+<table border=1>
-<tr><th></th><th>dH2O(ul)</th><th>Buffer(ul)</th><th>BSA(ul)</th><th>DNA(ul)</th><th>E(ul)</th><th>X(ul)</th><th>X(ul)</th><th>S(ul)</th><th>P(ul)</th></tr>
+<tr align="center">
-<tr><td>BBa_Elowitz</td><td>32.5</td><td>EcoRI, 5</td><td>0.5</td><td>10</td><td>1</td><td>-</td><td>1</td><td>-</td></tr>
+<th></th><th>dH2O(ul)</th><th>Buffer(ul)</th><th>BSA(ul)</th><th>DNA(ul)</th><th>E(ul)</th><th>X(ul)</th><th>S(ul)</th><th>P(ul)</th>
-<tr><td>LuxR</td><td>32.5</td><td>NEB3, 5</td><td>0.5</td><td>10</td><td>-</td><td>1</td><td>-</td><td>1</td></tr>
+</tr>
-<tr><td>GFP</td><td>32.5</td><td>NEB3, 5</td><td>0.5</td><td>10</td><td>-</td><td>1</td><td>-</td><td>1</td></tr>
+<tr align="center">
-<tr><td>P1010</td><td>32.5</td><td>EcoRI, 5</td><td>0.5</td><td>10</td><td>1</td><td>-</td><td>-</td><td>1</td></tr>
+<td>BBa_Elowitz</td><td>32.5</td><td>EcoRI, 5</td><td>0.5</td><td>10</td><td>1</td><td>-</td><td>1</td><td>-</td>
+</tr>
+<tr align="center">
+<td>LuxR</td><td>32.5</td><td>NEB3, 5</td><td>0.5</td><td>10</td><td>-</td><td>1</td><td>-</td><td>1</td>
+</tr>
+<tr align="center">
+<td>GFP</td><td>32.5</td><td>NEB3, 5</td><td>0.5</td><td>10</td><td>-</td><td>1</td><td>-</td><td>1</td>
+</tr>
+<tr align="center">
+<td>P1010</td><td>32.5</td><td>EcoRI, 5</td><td>0.5</td><td>10</td><td>1</td><td>-</td><td>-</td><td>1</td>
+</tr>
 </table>