Team:Paris/August 15

From 2008.igem.org

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(Starting the construction of the Promoter characterization plasmid)
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=Starting the construction of the Promoter characterization plasmid=
[[Team:Paris/Notebook/Protocols#Migration after digestion|standard protocol]]
[[Team:Paris/Notebook/Protocols#Migration after digestion|standard protocol]]
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===Digestion===
===Digestion===

Revision as of 15:52, 15 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Contents

Transformation of the ligations we did yesterday

We transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells
standard protocol.


Digestion

Measurement of concentration of minipreps

standard protocol

Digestion Miniprep used Concentration (µg/mL) ratio 260/280
D146 MP148.2 123 1.58
D147 MP153.3 113 1.68


Digestion

Protocol Digestion


Starting the construction of the Promoter characterization plasmid

standard protocol

Digestion

Measurement of concentration of minipreps

standard protocol

Digestion Miniprep used Concentration (µg/mL) ratio 260/280
D146 MP148.2 123 1.58
D147 MP153.3 113 1.68

Digestion

Protocol Digestion

Digestion Miniprep used Concentration (µg/mL) ratio 260/280
D146 MP148.2 123 1.58
D147 MP153.3 113 1.68

Analysis of yesterday PCR screening

KR000163.jpg KR000162.jpg KR000164.jpg