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Team:Paris/August 15
From 2008.igem.org
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===Digestion=== | ===Digestion=== | ||
[[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]] | [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]] | ||
| + | |||
| + | |||
| + | ==Ligation== | ||
| + | [[Team:Paris/Notebook/Protocols#Ligation|Protocol Ligation]] | ||
| + | |||
| + | {| border="1" style="text-align : center" | ||
| + | |Ligation Name | ||
| + | |Insert | ||
| + | |Vector | ||
| + | |- | ||
| + | |L143 | ||
| + | |D146 (strongest rbs-TetR-GFP tripart) | ||
| + | |D105 (pLas) | ||
| + | |- | ||
| + | |L144 | ||
| + | |D147 (strongest rbs-LasR activator with LVA) | ||
| + | |D125 (Double terminator) | ||
| + | |} | ||
==Analysis of yesterday PCR screening== | ==Analysis of yesterday PCR screening== | ||
Revision as of 09:32, 16 August 2008
Transformation of the ligations we did yesterdayWe transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells
DigestionMeasurement of concentration of minipreps
Digestion
Ligation
Analysis of yesterday PCR screening
Starting the construction of the Promoter characterization plasmidDigestionMeasurement of concentration of minipreps
Digestion
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