Team:MIT/Colony PCR

From 2008.igem.org

(Difference between revisions)
(New page: Colony PCR 2 uL primer #1 (to 10uM) 2 uL primer #2 0.4 uL DNTPs 2 uL Buffer 5 uL colony culture 0.2 uL Taq 10.4 uL H20 92C 1:30 (92 0:30 50 :30 72 1:30) repeat x30 72 10:00)
Line 2: Line 2:
2 uL primer #1 (to 10uM)
2 uL primer #1 (to 10uM)
 +
2 uL primer #2
2 uL primer #2
 +
0.4 uL DNTPs
0.4 uL DNTPs
 +
2 uL Buffer
2 uL Buffer
 +
5 uL colony culture
5 uL colony culture
 +
0.2 uL Taq
0.2 uL Taq
 +
10.4 uL H20
10.4 uL H20

Revision as of 20:12, 15 August 2008

Colony PCR

2 uL primer #1 (to 10uM)

2 uL primer #2

0.4 uL DNTPs

2 uL Buffer

5 uL colony culture

0.2 uL Taq

10.4 uL H20

92C 1:30 (92 0:30 50 :30 72 1:30) repeat x30 72 10:00