Alberta NINT/15 August 2008
From 2008.igem.org
(Difference between revisions)
Line 5: | Line 5: | ||
lab work (JD): | lab work (JD): | ||
Gel purification of TA2,TA3, TA6, TA7/B+N | Gel purification of TA2,TA3, TA6, TA7/B+N | ||
+ | |||
+ | lab work (SD): | ||
+ | Quantitated pSB1A3.1,.2, and .3 | ||
+ | Digested pSB1A3 with EcoRI+PstI | ||
+ | Incubated for 2 hrs at 37 C | ||
+ | Ran on 2% agarose gel (110 V for 60 min) | ||
+ | Excised fragments for pSB1A3.1 and pSB1A3.3 | ||
[[Alberta_NINT/16_August_2008 | Next entry >]] | [[Alberta_NINT/16_August_2008 | Next entry >]] |
Revision as of 21:00, 19 August 2008
lab work (JD):
Gel purification of TA2,TA3, TA6, TA7/B+N
lab work (SD):
Quantitated pSB1A3.1,.2, and .3 Digested pSB1A3 with EcoRI+PstI Incubated for 2 hrs at 37 C Ran on 2% agarose gel (110 V for 60 min) Excised fragments for pSB1A3.1 and pSB1A3.3