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Team:KULeuven/14 August 2008
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* The PCR sampeles of the T7 polymerase with UmuD tag were put on gel. The results were not good. | * The PCR sampeles of the T7 polymerase with UmuD tag were put on gel. The results were not good. | ||
* Some more ligation products were cut: J23109 + J23032, E0022 + B0015, C0062 + B0015 with ''Xba''I and ''EcoR''I; R0084 + J23022, R0062 + B0032, J23100 + B0032 with ''Spe''I and ''EcoR''I. Only R0062 + B0032 and J23100 + B0032 were not properly cut. It is possible thet the ligations are not correct. | * Some more ligation products were cut: J23109 + J23032, E0022 + B0015, C0062 + B0015 with ''Xba''I and ''EcoR''I; R0084 + J23022, R0062 + B0032, J23100 + B0032 with ''Spe''I and ''EcoR''I. Only R0062 + B0032 and J23100 + B0032 were not properly cut. It is possible thet the ligations are not correct. | ||
| - | * Ligations that failed were set up again: C0040 + B0015, GFP + B0015, C0012 + B0015, | + | * Ligations that failed were set up again: C0040 + B0015, GFP + B0015, C0012 + B0015, C0060 + B0015. |
=== Dry Lab === | === Dry Lab === | ||
Revision as of 10:46, 17 August 2008
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Contents |
Lab Work
Wet Lab
- The PCR sampeles of the T7 polymerase with UmuD tag were put on gel. The results were not good.
- Some more ligation products were cut: J23109 + J23032, E0022 + B0015, C0062 + B0015 with XbaI and EcoRI; R0084 + J23022, R0062 + B0032, J23100 + B0032 with SpeI and EcoRI. Only R0062 + B0032 and J23100 + B0032 were not properly cut. It is possible thet the ligations are not correct.
- Ligations that failed were set up again: C0040 + B0015, GFP + B0015, C0012 + B0015, C0060 + B0015.
Dry Lab
Modeling
Nick transferred all his assets to us today. He delegated heavily and provided us with workable stuff for next week :) The wiki was also updated and remarks are all around, so a lot of work still to do
Today, we implemented auto-activation of the Lux repressor, mimicking the original Vibrio Lux system. After a lot of messing about and a lot of long kinetic laws we finally managed it and it seems to be working correctly. It's very hard though to keep track of all the correct/changed parameters in such a large system :/
It's been said before this week, but things are looking quite good. We might have reached some sort of end point here where everything should be final. (hopefully)
Anyhow, time for a long weekend. See you on monday!
Wiki
Homepage code reformatted, and content added. Maarten made a image map of the team picture. IE fixes for tabs and picture gallery added.
