Team:Chiba/Calendar-Home/17 August 2008

From 2008.igem.org

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===Output team===
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>[[Team:Chiba/Home|Home]]
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バイオブリックパーツおこし
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<br>BBa_I73002
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<br>BBa_I73004
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<br>BBa_I73003
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<br>BBa_I763026
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<br>BBa_E0430
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<br>BBa_E0420
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<br>BBa_J60720
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<br>BBa_S01416
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<br>Elution :EB buffer 5μL -> Spin(15000rpm,3Min.)
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Insert PCR
 
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<br>DNA template 1μL
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[[Team:Chiba/Calendar-Home/16 August 2008|16 August 2008]]<|>[[Team:Chiba/Calendar-Home/19 August 2008|19 August 2008]]
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<br>Fwd primer(VF II) 5μL
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<br>Rev primer(VR)    5μL
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<br>Termo pol buffer  10μL
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<br>Vent pol 1μL
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<br>dH2O 68μL
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<br>==========================
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<br>Total:100μL
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 +
==Laboratory work==
 +
===Team:Input===
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no work
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95℃(5min.) -> [95℃(30sec.) -> 53℃(1min.) -> 72℃(2min.)]x25cycle -> 72℃(10min.) -> store
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===Team:Communication===
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:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
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:competent cells : XL10G
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::*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040(2008)] -> No colonies on plates
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::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070(2008)] -> No colonies on plates
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::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076(2008)] -> No colonies on plates
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::*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077(2008)] -> No colonies on plates
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::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078(2008)] -> No colonies on plates
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::*[http://partsregistry.org/Part:BBa_C0079 BBa_C0079(2008)] -> No colonies on plates
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Gel check
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 +
 
 +
===Team:Output===
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'''[[Team:Chiba/protocol/PCR|PCR]]'''
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*[http://partsregistry.org/Part:BBa_E0430 BBa_E0430]①
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*[http://partsregistry.org/Part:BBa_E0420 BBa_E0420]②
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*[http://partsregistry.org/Part:BBa_J06702 BBa_J06702]③
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*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]④
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*BBa_I73003⑤
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*BBa_I73004⑥
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*BBa_I730026⑦
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*[http://partsregistry.org/Part:BBa_I763002 BBa_I763002]⑧
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<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample No.</td>
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<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td><td>⑦</td><td>⑧</td>
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</tr>
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<tr>
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<td>DNA tamplate</td>
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<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td><td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>FW primer</td>
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<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
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</tr>
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<tr>
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<td>VR primer</td>
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<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
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</tr>
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<tr>
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<td>dNTPmix</td>
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<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
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</tr>
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<tr>
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<td>thermo pol buffer</td>
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<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
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</tr>
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<tr>
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<td>DNA pol(VENT)</td>
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<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>dH<sub>2</sub>O</td>
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<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
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</tr>
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</table>

Revision as of 06:41, 24 October 2008

>Home


16 August 2008<|>19 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

Transformation
competent cells : XL10G
  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040(2008)] -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070(2008)] -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076(2008)] -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0077 BBa_C0077(2008)] -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078(2008)] -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0079 BBa_C0079(2008)] -> No colonies on plates


Team:Output

PCR

  • [http://partsregistry.org/Part:BBa_E0430 BBa_E0430]①
  • [http://partsregistry.org/Part:BBa_E0420 BBa_E0420]②
  • [http://partsregistry.org/Part:BBa_J06702 BBa_J06702]③
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]④
  • BBa_I73003⑤
  • BBa_I73004⑥
  • BBa_I730026⑦
  • [http://partsregistry.org/Part:BBa_I763002 BBa_I763002]⑧
>
Sample No. ①②③④⑤⑥⑦⑧
DNA tamplate 11111111
FW primer 55555555
VR primer 55555555
dNTPmix 1010101010101010
thermo pol buffer 1010101010101010
DNA pol(VENT) 11111111
dH2O 6868686868686868
TOTAL 100100100100100100100100
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/17_August_2008"