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Team:Paris/Modeling/f2
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| - | [[Image:f2.jpg]] | + | [[Image:f2.jpg|thumb]] According to previous consideration on pBad, we have |
| + | |||
| + | <center>[[Image:f2expr.jpg]]</center> | ||
| + | |||
| + | so, with the complexation between Arabinose and AraC, and after reducing the eaquation | ||
| + | |||
| + | <center>[[Image:f2exprRedu.jpg]]</center> | ||
| + | |||
| + | |||
| + | {|border="1" style="text-align: center" | ||
| + | |param | ||
| + | |signification | ||
| + | |unit | ||
| + | |value | ||
| + | |- | ||
| + | |[expr(pTet)] | ||
| + | |expression rate of <br> pTet '''with RBS E0032''' | ||
| + | |nM.s<sup>-1</sup> | ||
| + | |see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 measures | ||
| + | |- | ||
| + | |γ<sub>GFP</sub> | ||
| + | |dilution-degradation rate <br> of GFP(mut3b) | ||
| + | |s<sup>-1</sup> | ||
| + | |ln(2)/3600 | ||
| + | |- | ||
| + | |[GFP] | ||
| + | |GFP concentration at steady-state | ||
| + | |nM | ||
| + | |need for 20 measures | ||
| + | |- | ||
| + | |(''fluorescence'') | ||
| + | |value of the observed fluorescence | ||
| + | |au | ||
| + | |need for 20 measures | ||
| + | |- | ||
| + | |''conversion'' | ||
| + | |conversion ration between <br> fluorescence and concentration | ||
| + | |nM.au<sup>-1</sup> | ||
| + | |(1/79.429) | ||
| + | |} | ||
| + | |||
| + | <br><br> | ||
| + | |||
| + | {|border="1" style="text-align: center" | ||
| + | |param | ||
| + | |signification <br> corresponding parameters in the [[Team:Paris/Modeling/Oscillations#Resulting_Equations|equations]] | ||
| + | |unit | ||
| + | |value | ||
| + | |- | ||
| + | |β<sub>tet</sub> | ||
| + | |production rate of pTet '''with RBS E0032''' <br> β<sub>1</sub> | ||
| + | |nM.s<sup>-1</sup> | ||
| + | | | ||
| + | |- | ||
| + | |(K<sub>tet</sub>/{coef<sub>tetR</sub>}<sup>n<sub>tet</sub></sup>) | ||
| + | |activation constant of pTet <br> K<sub>18</sub> | ||
| + | |nM<sup>n<sub>tet</sub></sup> | ||
| + | | | ||
| + | |- | ||
| + | |n<sub>tet</sub> | ||
| + | |complexation order of pTet<br> n<sub>18</sub> | ||
| + | |no dimension | ||
| + | | | ||
| + | |- | ||
| + | |K<sub>aTc</sub> | ||
| + | |complexation constant aTc-TetR <br> K<sub>17</sub> | ||
| + | |nM<sup>n<sub>aTc</sub></sup> | ||
| + | | | ||
| + | |- | ||
| + | |n<sub>aTc</sub> | ||
| + | |complexation order aTc-TetR <br> n<sub>17</sub> | ||
| + | |no dimension | ||
| + | | | ||
| + | |} | ||
Revision as of 14:02, 21 August 2008
so, with the complexation between Arabinose and AraC, and after reducing the eaquation
| param | signification | unit | value |
| [expr(pTet)] | expression rate of pTet with RBS E0032 | nM.s-1 | see "findparam" need for 20 measures |
| γGFP | dilution-degradation rate of GFP(mut3b) | s-1 | ln(2)/3600 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 measures |
| (fluorescence) | value of the observed fluorescence | au | need for 20 measures |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| βtet | production rate of pTet with RBS E0032 β1 | nM.s-1 | |
| (Ktet/{coeftetR}ntet) | activation constant of pTet K18 | nMntet | |
| ntet | complexation order of pTet n18 | no dimension | |
| KaTc | complexation constant aTc-TetR K17 | nMnaTc | |
| naTc | complexation order aTc-TetR n17 | no dimension |
