Team:NTU-Singapore/Notebook/29 May 2008

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==Wednesday==
==Wednesday==
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*ChinChong (~~)  
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===ChinChong (~~)===
**Requested E coli strain B2B110 with the ColE7 plasmid from Professor David Gordon (Australia)
**Requested E coli strain B2B110 with the ColE7 plasmid from Professor David Gordon (Australia)
**Requested quotation from Invitrogen on some chemicals and kits
**Requested quotation from Invitrogen on some chemicals and kits
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**Finding parameters for the modeling from literature
**Finding parameters for the modeling from literature
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*Darius
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===Darius===
**Come up with provisional LacI experiment design
**Come up with provisional LacI experiment design
**Discussed possible experiments design to patch up the parts of our BioBrick
**Discussed possible experiments design to patch up the parts of our BioBrick
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*Choon Kit
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===Choon Kit===
**Plasmid Extraction using Qiagen Miniprep. Ligation using EcoRI and PstI  
**Plasmid Extraction using Qiagen Miniprep. Ligation using EcoRI and PstI  
**Alias with Professor Yuan Hanna (Taiwan)to get E7+Imm plasmid, expected arrival 5th Jun 08
**Alias with Professor Yuan Hanna (Taiwan)to get E7+Imm plasmid, expected arrival 5th Jun 08
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**Documented laboratory work for 28 and 29 May 2008  
**Documented laboratory work for 28 and 29 May 2008  
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*Hung
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===Hung===
**Plasmid Extraction using Qiagen Miniprep. Ligation using EcoRI and PstI (basically phase II and up to step 3 of phase III of experiment 3 for iron promoter. We left the DNAs incubated overnight instead of for 2hrs due to time constraint.)
**Plasmid Extraction using Qiagen Miniprep. Ligation using EcoRI and PstI (basically phase II and up to step 3 of phase III of experiment 3 for iron promoter. We left the DNAs incubated overnight instead of for 2hrs due to time constraint.)
**Finding parameters for the modeling from literature
**Finding parameters for the modeling from literature
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*ZhenFu
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===ZhenFu===
**Improve our group's model with some literature parameters
**Improve our group's model with some literature parameters
**Added equations to our model
**Added equations to our model
**Puzzled over results of simulation, shall work on it further with other members of the group
**Puzzled over results of simulation, shall work on it further with other members of the group
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Revision as of 04:10, 25 October 2008

Contents

Wednesday

ChinChong (~~)

    • Requested E coli strain B2B110 with the ColE7 plasmid from Professor David Gordon (Australia)
    • Requested quotation from Invitrogen on some chemicals and kits
    • Discussed possible experiments design to patch up the parts of our BioBrick
    • Finding parameters for the modeling from literature

Darius

    • Come up with provisional LacI experiment design
    • Discussed possible experiments design to patch up the parts of our BioBrick

Choon Kit

    • Plasmid Extraction using Qiagen Miniprep. Ligation using EcoRI and PstI
    • Alias with Professor Yuan Hanna (Taiwan)to get E7+Imm plasmid, expected arrival 5th Jun 08
    • Alias with Professor Chris Viogt (UCSF) to get AND modular plasmids, expected arrival 5th Jun 08
    • Requested full DNA sequence of ColE7 and Imm plasmid from Zhong Zhi Hao (Taiwan)
    • Requested full DNA sequence of AND modular plasmids from Chris Voigt (UCSF)
    • Documented laboratory work for 28 and 29 May 2008

Hung

    • Plasmid Extraction using Qiagen Miniprep. Ligation using EcoRI and PstI (basically phase II and up to step 3 of phase III of experiment 3 for iron promoter. We left the DNAs incubated overnight instead of for 2hrs due to time constraint.)
    • Finding parameters for the modeling from literature

ZhenFu

    • Improve our group's model with some literature parameters
    • Added equations to our model
    • Puzzled over results of simulation, shall work on it further with other members of the group
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