User:University of Washington/20 August 2008
From 2008.igem.org
(Difference between revisions)
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-picked 4 colonies and streaked out on Amp, Cam, Kan, and Tet plates. | -picked 4 colonies and streaked out on Amp, Cam, Kan, and Tet plates. | ||
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+ | ==LuxR from pLac== | ||
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+ | -Minipreps of parts R0010 and I0462 were digested for assembly and ran on a gel to separate fragments. | ||
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+ | -The R0010 vector and I0462 insert fragments were excised from the gel. | ||
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Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] |
Latest revision as of 21:57, 22 August 2008
Contents |
LuxR from AraC and TetR(Faifan)
Elowitz
- ran gel for digested P1010(KAN), E0240, I0462, BBa_Elowitz
- cut out the bands and did Gel purification
MG1655Z1(Faifan)
-received culture on non-selective plate from Ingrid
-picked 4 colonies and streaked out on Amp, Cam, Kan, and Tet plates.
LuxR from pLac
-Minipreps of parts R0010 and I0462 were digested for assembly and ran on a gel to separate fragments.
-The R0010 vector and I0462 insert fragments were excised from the gel.
Back to Team:University_of_Washington/Notebook#Notebook