Team:Paris/Modeling/f2

From 2008.igem.org

(Difference between revisions)
Line 3: Line 3:
<center>[[Image:f2expr.jpg]]</center>
<center>[[Image:f2expr.jpg]]</center>
-
so, with the complexation between Arabinose and AraC, and after reducing the eaquation
+
so, with the complexation between Arabinose and AraC, and after reducing the equation
<center>[[Image:f2exprRedu.jpg]]</center>
<center>[[Image:f2exprRedu.jpg]]</center>
Line 14: Line 14:
|value
|value
|-
|-
-
|[expr(pTet)]
+
|[expr(pBad)]
-
|expression rate of <br> pTet '''with RBS E0032'''
+
|expression rate of <br> pBad '''with RBS E0032'''
|nM.s<sup>-1</sup>
|nM.s<sup>-1</sup>
|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 measures
|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 measures
Line 48: Line 48:
|value
|value
|-
|-
-
|β<sub>tet</sub>
+
|β<sub>bad</sub>
-
|production rate of pTet '''with RBS E0032''' <br> β<sub>1</sub>
+
|production rate of pBad '''with RBS E0032''' <br> not in the system
|nM.s<sup>-1</sup>
|nM.s<sup>-1</sup>
|
|
|-
|-
-
|(K<sub>tet</sub>/{coef<sub>tetR</sub>}<sup>n<sub>tet</sub></sup>)
+
|(K<sub>bad</sub>/[AraC<sub>tot</sub>]<sup>n<sub>bad</sub></sup>)
-
|activation constant of pTet <br> K<sub>18</sub>
+
|activation constant of pBad <br> not in the system
-
|nM<sup>n<sub>tet</sub></sup>
+
|nM<sup>n<sub>bad</sub></sup>
|
|
|-
|-
-
|n<sub>tet</sub>
+
|n<sub>bad</sub>
-
|complexation order of pTet<br> n<sub>18</sub>
+
|complexation order of pBad<br> not in the system
|no dimension
|no dimension
|
|
|-
|-
-
|K<sub>aTc</sub>
+
|K<sub>ara</sub>
-
|complexation constant aTc-TetR <br> K<sub>17</sub>
+
|complexation constant Arabinose-AraC <br> not in the system
-
|nM<sup>n<sub>aTc</sub></sup>
+
|nM<sup>n<sub>ara</sub></sup>
|
|
|-
|-
-
|n<sub>aTc</sub>
+
|n<sub>ara</sub>
-
|complexation order aTc-TetR <br> n<sub>17</sub>
+
|complexation order Arabinose-AraC <br> n<sub>17</sub>
|no dimension
|no dimension
|
|
|}
|}

Revision as of 14:09, 21 August 2008

F2.jpg
According to previous consideration on pBad, we have
F2expr.jpg

so, with the complexation between Arabinose and AraC, and after reducing the equation

F2exprRedu.jpg


param signification unit value
[expr(pBad)] expression rate of
pBad with RBS E0032 		nM.s-1 see "findparam"
need for 20 measures
γGFP dilution-degradation rate
of GFP(mut3b) 		s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 measures
(fluorescence) value of the observed fluorescence au need for 20 measures
conversion conversion ration between
fluorescence and concentration 		nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations 		unit value
βbad production rate of pBad with RBS E0032
not in the system 		nM.s-1
(Kbad/[AraCtot]nbad) activation constant of pBad
not in the system 		nMnbad
nbad complexation order of pBad
not in the system 		no dimension
Kara complexation constant Arabinose-AraC
not in the system 		nMnara
nara complexation order Arabinose-AraC
n17 		no dimension
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