"
Team:Paris/Modeling/f3
From 2008.igem.org
(Difference between revisions)
| Line 31: | Line 31: | ||
|[expr(pFlhDC)] | |[expr(pFlhDC)] | ||
|expression rate of <br> pFlhDC '''with RBS E0032''' | |expression rate of <br> pFlhDC '''with RBS E0032''' | ||
| - | |nM. | + | |nM.min<sup>-1</sup> |
|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''? | |see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''? | ||
|- | |- | ||
|γ<sub>GFP</sub> | |γ<sub>GFP</sub> | ||
|dilution-degradation rate <br> of GFP(mut3b) | |dilution-degradation rate <br> of GFP(mut3b) | ||
| - | | | + | |min<sup>-1</sup> |
| - | | | + | |0.0198 |
|- | |- | ||
|[GFP] | |[GFP] | ||
| Line 65: | Line 65: | ||
|β<sub>12</sub> | |β<sub>12</sub> | ||
|production rate of FliA-pFlhDC '''with RBS E0032''' <br> β<sub>12</sub> | |production rate of FliA-pFlhDC '''with RBS E0032''' <br> β<sub>12</sub> | ||
| - | |nM. | + | |nM.min<sup>-1</sup> |
| | | | ||
|- | |- | ||
| - | |(K<sub>11</sub>/{coef<sub>fliA</sub>} | + | |(K<sub>11</sub>/{coef<sub>fliA</sub>}) |
|activation constant of FliA-pFlhDC <br> K<sub>11</sub> | |activation constant of FliA-pFlhDC <br> K<sub>11</sub> | ||
| - | |nM | + | |nM |
| | | | ||
|- | |- | ||
| Line 84: | Line 84: | ||
| | | | ||
|- | |- | ||
| - | |(K<sub>19</sub>/{coef<sub>ompR</sub>} | + | |(K<sub>19</sub>/{coef<sub>ompR</sub>}) |
|activation constant of OmpR-pFlhDC <br> K<sub>19</sub> | |activation constant of OmpR-pFlhDC <br> K<sub>19</sub> | ||
| - | |nM | + | |nM |
| | | | ||
|- | |- | ||
Revision as of 22:26, 4 September 2008
and
so the expression
gives
and
and for calculated values of the TF,
and
| param | signification | unit | value |
| [expr(pFlhDC)] | expression rate of pFlhDC with RBS E0032 | nM.min-1 | see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM? |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the SUM? |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the SUM? |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| β12 | production rate of FliA-pFlhDC with RBS E0032 β12 | nM.min-1 | |
| (K11/{coeffliA}) | activation constant of FliA-pFlhDC K11 | nM | |
| n11 | complexation order of FliA-pFlhDC n11 | no dimension | |
| β2 | production rate of OmpR-pFlhDC with RBS E0032 β2 | nM.s-1 | |
| (K19/{coefompR}) | activation constant of OmpR-pFlhDC K19 | nM | |
| n19 | complexation order of OmpR-pFlhDC n19 | no dimension |
Then, if we have time, we want to verify the expected relation
