Team:Paris/Modeling/f8

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[[Image:f8DCA.png|thumb]]
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[[Image:f8DCA.png|thumb]] At the steady-state, we have
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<center>[[Image:FlhDCeq.jpg]]</center>
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and
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<center>[[Image:FliAeq.jpg]]</center>
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so the expression
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<center>[[Image:f8expr.jpg]]</center>
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gives
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<center>[[Image:f8FlhDC.jpg]]</center>
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and
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<center>[[Image:f8FliA.jpg]]</center>
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and for ''calculated values of the TF'',
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<center>[[Image:f8FlhDCCalc.jpg]]</center>
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and
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<center>[[Image:f8FliACalc.jpg]]</center>
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<br><br>
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{|border="1" style="text-align: center"
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|param
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|signification
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|unit
 +
|value
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|-
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|[expr(pFlhB)]
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|expression rate of <br> pFlhB '''with RBS E0032'''
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|nM.s<sup>-1</sup>
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|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|-
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|γ<sub>GFP</sub>
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|dilution-degradation rate <br> of GFP(mut3b)
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|s<sup>-1</sup>
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|ln(2)/3600
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|-
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|[GFP]
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|GFP concentration at steady-state
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|nM
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|need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|-
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|(''fluorescence'')
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|value of the observed fluorescence
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|au
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|need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|-
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|''conversion''
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|conversion ration between <br> fluorescence and concentration
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|nM.au<sup>-1</sup>
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|(1/79.429)
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|}
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<br><br>
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{|border="1" style="text-align: center"
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|param
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|signification <br> corresponding parameters in the [[Team:Paris/Modeling/Oscillations#Resulting_Equations|equations]]
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|unit
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|value
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|-
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|β<sub>57</sub>
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|production rate of FlhDC-pFlgB '''with RBS E0032''' <br> β<sub>57</sub>
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|nM.s<sup>-1</sup>
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|
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|-
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|(K<sub>51</sub>/{coef<sub>flhDC</sub>}<sup>n<sub>51</sub></sup>)
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|activation constant of FlhDC-pFlgB <br> K<sub>51</sub>
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|nM<sup>n<sub>51</sub></sup>
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|
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|-
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|n<sub>51</sub>
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|complexation order of FliA-pFlgB <br> n<sub>51</sub>
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|no dimension
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|
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|-
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|-
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|β<sub>58</sub>
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|production rate of FliA-pFlgB '''with RBS E0032''' <br> β<sub>52</sub>
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|nM.s<sup>-1</sup>
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|
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|-
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|(K<sub>52</sub>/{coef<sub>fliA</sub>}<sup>n<sub>52</sub></sup>)
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|activation constant of FliA-pFlgA <br> K<sub>52</sub>
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|nM<sup>n<sub>52</sub></sup>
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|
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|-
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|n<sub>52</sub>
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|complexation order of FliA-pFlgB <br> n<sub>52</sub>
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|no dimension
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|
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|}
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<br><br>
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Then, if we have time, we want to verify the expected relation
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<center>[[Image:SumpFlhB.jpg]]</center>

Revision as of 17:44, 21 August 2008

F8DCA.png
At the steady-state, we have
FlhDCeq.jpg

and

FliAeq.jpg

so the expression

F8expr.jpg

gives

F8FlhDC.jpg

and

F8FliA.jpg

and for calculated values of the TF,

F8FlhDCCalc.jpg

and

F8FliACalc.jpg



param signification unit value
[expr(pFlhB)] expression rate of
pFlhB with RBS E0032 		nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b) 		s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration 		nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations 		unit value
β57 production rate of FlhDC-pFlgB with RBS E0032
β57 		nM.s-1
(K51/{coefflhDC}n51) activation constant of FlhDC-pFlgB
K51 		nMn51
n51 complexation order of FliA-pFlgB
n51 		no dimension
β58 production rate of FliA-pFlgB with RBS E0032
β52 		nM.s-1
(K52/{coeffliA}n52) activation constant of FliA-pFlgA
K52 		nMn52
n52 complexation order of FliA-pFlgB
n52 		no dimension



Then, if we have time, we want to verify the expected relation

SumpFlhB.jpg
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