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Alberta NINT/21 August 2008
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Ligated TA2+(PCR)/B+N (NR) into K102031/B+N (GP). | Ligated TA2+(PCR)/B+N (NR) into K102031/B+N (GP). | ||
Transformed into XL1-B cells and incubated overnight at 37 C. | Transformed into XL1-B cells and incubated overnight at 37 C. | ||
| + | |||
| + | lab work (JD): | ||
| + | Digested mini-prep of cotransformed cells with EcoR1 | ||
| + | -to run on gel and check if in fact both plasmids inside the cell are low copy plasmids, bands should be about the same brightness | ||
| + | Ran on gel | ||
| + | Analyzed LacZ Assay Data | ||
| + | |||
[[Alberta_NINT/22_August_2008 | Next entry >]] | [[Alberta_NINT/22_August_2008 | Next entry >]] | ||
Revision as of 02:54, 4 September 2008
lab work (SD):
Ligated TA2+(PCR)/B+N (NR) into K102031/B+N (GP).
Transformed into XL1-B cells and incubated overnight at 37 C.
lab work (JD):
Digested mini-prep of cotransformed cells with EcoR1
-to run on gel and check if in fact both plasmids inside the cell are low copy plasmids, bands should be about the same brightness
Ran on gel
Analyzed LacZ Assay Data
