Team:BCCS-Bristol/Protocols-Media

From 2008.igem.org

(Difference between revisions)
(→Media)
(→LB broth or agar (200 ml))
Line 25: Line 25:
| align="center" style="background:#f0f0f0;"|'''Concentration [%]'''
| align="center" style="background:#f0f0f0;"|'''Concentration [%]'''
| align="center" style="background:#f0f0f0;"|'''Amount for 200 ml'''
| align="center" style="background:#f0f0f0;"|'''Amount for 200 ml'''
-
|-
 
-
| ||||||
 
|-
|-
|Yeast Extract||0.5||1 g
|Yeast Extract||0.5||1 g

Revision as of 08:54, 25 August 2008

Home The Team The Project Submitted Parts Modelling Wet Lab Calendar Miscellaneous


Media

LB broth or agar (200 ml)

Component Concentration [%] Amount for 200 ml
Yeast Extract0.51 g
Bactotryptone12 g
NaCl12 g
AgarFor plates 2For plates 4 g

Swimming agar medium

Component Concentration [%] Amount for 200 ml
Bactotryptone12 g
NaCl0.52 g
Agarvarious

SOC medium

Component Concentration [%] Amount for 95 (100) ml
Bactotryptone22 g
Bacto yeast extract0.50.5 g
NaCl0.050.05 g

Mix the three components with 95 ml distilled water and autoclave it. Just before using, add (filter sterilized):

• 0.5 ml 2 M MgCl2

• 2 ml 1 M Glucose


Retrieved from "http://2008.igem.org/Team:BCCS-Bristol/Protocols-Media"