Team:Johns Hopkins/Protocols

Protocols

PCR Protocols from Jaime
Ligation/Cloning and Transformation
Moodle Colony Screening PCR
Plasmid Miniprep
Checking Minipreps to Prepare for Sequencing
Sequence Sample Submission
Making Glycerol Stocks
Moving Insert to iGEM Vector

Papers

Important Papers

Reshma P Shetty, Drew Endy, and Thomas F Knight Jr; Engineering BioBrick vectors from BioBrick parts

*d Herskowitz I. A regulatory hierarchy for cell specialization in yeast. (REVIEW.) Nature. 342:749-757 (1989)

1 Johnson AD. Molecular mechanisms of cell-type determination in budding yeast. (REVIEW.) Current Opinion in Gen. & Dev. 5:552-558 (1995)

1 Hu R-G et al. The N-end rule pathway as a nitric oxide sensor controlling the levels of multiple regulators. Nature. 437:981-986 (2005)

1 Varshavsky A. The N-end Rule pathway of protein degradation. (REVIEW.) Genes to Cells. 2:13-28 (1997)

1 Sheff MA & Thorn KS. Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast. 21:661-670 (2004)

1 Bardwell L. A walk-through of the yeast mating pheromone response pathway. (REVIEW.) Peptides. 25:1465-1476 (2004)

Yeast Vector Papers

**Broach JR. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene. 8(1):121-133 (1979)

** Stearns, T. Manipulating yeast genome using plasmid vectors. (REVIEW.) Methods in enzymology. 185:280-297 (1990)

1 Hill JE et al. Yeast/E. coli Shuttle Vectors with Multiple Unique Restriction Sites. Yeast. 2:163-167 (1986)

** Sikorski RS. A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122:19-27 (1989)

*** Mumberg D et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene. 156:119-122 (1995)

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