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Team:University of Lethbridge/Notebook/Project2September
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[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]] | [[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]] | ||
| + | |||
| + | ===September 1, 2008=== | ||
| + | ====Roxanne==== | ||
| + | -Picked the colonies which appear to have amplified during the screening PCR, and Incubated them in LB+amp media at 37.0C overnight. | ||
| + | |||
| + | ===September 2, 2008=== | ||
| + | ====Roxanne==== | ||
| + | -Plasmid Prepped the colonies which were incubated overnight. Ran Plasmids on a 1% Agarose Gel at 100V for 27 minutes. | ||
| + | |||
| + | ====Nathan Puhl==== | ||
| + | -Visulaized the Gel, and chose appropriate cells to glycerol stock. | ||
| + | |||
| + | ====September 3, 2008==== | ||
| + | ===Roxanne, Nathan Puhl=== | ||
| + | -Performed a Restriction Digest on RS1 and RS2 from pGEM T-easy using XbaI and SpeI. Ran overnight at 37.0C | ||
Revision as of 23:36, 3 September 2008
Back to The University of Lethbridge Main Notebook
Contents |
September 1, 2008
Roxanne
-Picked the colonies which appear to have amplified during the screening PCR, and Incubated them in LB+amp media at 37.0C overnight.
September 2, 2008
Roxanne
-Plasmid Prepped the colonies which were incubated overnight. Ran Plasmids on a 1% Agarose Gel at 100V for 27 minutes.
Nathan Puhl
-Visulaized the Gel, and chose appropriate cells to glycerol stock.
September 3, 2008
Roxanne, Nathan Puhl
-Performed a Restriction Digest on RS1 and RS2 from pGEM T-easy using XbaI and SpeI. Ran overnight at 37.0C
