"
TUDelft/19 August 2008
From 2008.igem.org
(Difference between revisions)
(→Testing the Primers) |
(→Testing the Primers) |
||
| Line 7: | Line 7: | ||
==Testing the Primers== | ==Testing the Primers== | ||
| - | The PCR products were run on two different 1.5% agarose gels. Also a marker was run on the gel. The expected band sizes are ~550bp for IDI, ~900bp for ISPA and ~1200bp for ATOB. The pictures are shown below: | + | The PCR products were run on two different 1.5% agarose gels. Also a marker was run on the gel. The expected band sizes are ~550bp for IDI, ~900bp for ISPA and ~1200bp for ATOB. It is known that bands end up a little higher when running the SybrSafe product in the gel. The pictures are shown below: |
[[Image:gelTUDelft1.jpg|thumb|left|Gel of IDI and ISPA, click picture to enlarge]] | [[Image:gelTUDelft1.jpg|thumb|left|Gel of IDI and ISPA, click picture to enlarge]] | ||
Revision as of 08:58, 26 August 2008
[http://www.tudelft.nl ]
[http://www.tudelft.nl ]
19th August
Testing the Primers
The PCR products were run on two different 1.5% agarose gels. Also a marker was run on the gel. The expected band sizes are ~550bp for IDI, ~900bp for ISPA and ~1200bp for ATOB. It is known that bands end up a little higher when running the SybrSafe product in the gel. The pictures are shown below:
STILL NEED TO EDIT/PUT IN THE PICTURES
Live stabs
All the live stabs cultures have grown in 5 ml LB + A. We've made glycerol stocks, plated them and grown them in 50 ml LB medium + A o/n again to perform midiprep tomorrow.
