Team:Paris/August 26
From 2008.igem.org
(→Results of the electrophoresis) |
(→Extraction of EnvZ* and OmpR* from E. coli genome) |
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==Cleaning of the PCR products== | ==Cleaning of the PCR products== | ||
+ | [[Team:Paris/Notebook/Protocols#Purification_.28Kit_Promega.29|Standard protocol]] | ||
==Digestion of the PCR products== | ==Digestion of the PCR products== | ||
Revision as of 16:36, 26 August 2008
Extraction of EnvZ* and OmpR* from E. coli genomeName of the PCR
ProtocolProtocol
PCR Programme
ResutsElectrophoresis settings
Results of the electrophoresis
Cleaning of the PCR productsDigestion of the PCR productsPCRPCR1
Program: Gradient Vf=20µL PCR2
Program: promoter Vf=50µL PCR3
Program: PCRFliA1 Program: PCRFliA2 Program: PCRFliA3
Cloning of EnvZ*Concentration measurement by Biophotometer
Ligation
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