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Template:Team:UC Berkeley/Notebook/CDB construction
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ca8374 Reverse Biobricking of mRFP | ca8374 Reverse Biobricking of mRFP | ||
CGttaGGATCCttactcccccaagaaaatcc | CGttaGGATCCttactcccccaagaaaatcc | ||
| + | </pre> | ||
| + | |||
| + | ==pBca1256-K112400== | ||
| + | |||
| + | <pre> | ||
| + | Biobricking of promoter for grpE protein on MG1655 | ||
| + | PCR grpE-F and grpE-R (129 bp, EcoR1/BamH1/Dpn1) | ||
| + | Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) | ||
| + | Product is pBca1256-K112400 | ||
| + | ---------------------------- | ||
| + | grpE-F Forward Biobricking ccataGAATTCatgAGATCTccgaggtccttgttgcgaaga | ||
| + | grpE-R Reverse Biobricking cgttaGGATCCcgcattcatctcgctaacttc | ||
| + | |||
</pre> | </pre> | ||
Revision as of 18:35, 27 June 2008
PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112800 ----------------------------------------------- ca8373 Forward Biobricking of mRFP CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag ca8374 Reverse Biobricking of mRFP CGttaGGATCCttactcccccaagaaaatcc
pBca1256-K112400
Biobricking of promoter for grpE protein on MG1655 PCR grpE-F and grpE-R (129 bp, EcoR1/BamH1/Dpn1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112400 ---------------------------- grpE-F Forward Biobricking ccataGAATTCatgAGATCTccgaggtccttgttgcgaaga grpE-R Reverse Biobricking cgttaGGATCCcgcattcatctcgctaacttc
