"
Team:Chiba/protocol/ligation/gelex
From 2008.igem.org
(Difference between revisions)
(New page: >Protcol) |
|||
| Line 1: | Line 1: | ||
>[[Team:Chiba/protocol|Protcol]] | >[[Team:Chiba/protocol|Protcol]] | ||
| + | |||
| + | Gel extract | ||
| + | |||
| + | READ:Agarose gel electrophoresis | ||
| + | |||
| + | #Run the Digested DNA solution | ||
| + | #Cut the agarose taget band | ||
| + | #The chip of the gel into ADB buffer | ||
| + | #Let it in 37 degree 30 min to solve the agarose gel. | ||
| + | #Purify the DNA with Zymo clean column | ||
| + | |||
| + | READ:Zymo clean | ||
Revision as of 03:02, 11 September 2008
Gel extract
READ:Agarose gel electrophoresis
- Run the Digested DNA solution
- Cut the agarose taget band
- The chip of the gel into ADB buffer
- Let it in 37 degree 30 min to solve the agarose gel.
- Purify the DNA with Zymo clean column
READ:Zymo clean
