Template:Team:UC Berkeley/Notebook/BX construction

From 2008.igem.org

(Difference between revisions)
(→Sixpi 22:25, 4 June 2008 (UTC))
Line 56: Line 56:
<pre>
<pre>
-
PCR bx6/bx2 on Bjh1301 (701 bp, EcoRI/BamHI/DpnI)
+
PCR bx6/bx2 on Bjh1301 (704 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112005
Product is pBca1256-K112005
----------------------------------------
----------------------------------------
bx6 Forward Biobricking of {rbs.T4 Holin!}
bx6 Forward Biobricking of {rbs.T4 Holin!}
-
CCATAGAATTCatgAGATCTgaagaaaaaacatatggcagcacctagaat
+
CCATAGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc
bx2 Reverse Biobricking of {rbs.T4 Holin!}
bx2 Reverse Biobricking of {rbs.T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct
cgttaGGATCCttatttagcccttcctaatattct
Line 122: Line 122:
<pre>
<pre>
-
PCR bx12/bx8 on Bjh1303 (338 bp, EcoRI/BamHI/DpnI)
+
PCR bx12/bx8 on Bjh1303 (339 bp, EcoRI/BamHI/DpnI)
-
Sub into pBca1256 (EcoRI/BamHI)
+
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112011
Product is pBca1256-K112011
----------------------------------------
----------------------------------------
-
bx12 Forward biobricking of {rbs.T4 Antiholin!}
+
bx12 Forward biobricking of {rbs.T4 Antiholin!}
-
CCATAGAATTCatgAGATCTgaagaaaaaacatatggccttaaaagcaacagc
+
CCATAGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc
bx8 Reverse biobricking of {rbs.T4 Antiholin!}
bx8 Reverse biobricking of {rbs.T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg
cgttaGGATCCtcattcagtctccaatttaatg
Line 188: Line 188:
<pre>
<pre>
-
PCR bx18/bx16 on Bjh1302 (539 bp, EcoRI/BamHI/DpnI)
+
PCR bx18/bx16 on Bjh1302 (542 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112017
Product is pBca1256-K112017
----------------------------------------
----------------------------------------
bx18      Forward biobricking of {rbs.T4 Endolysin!}
bx18      Forward biobricking of {rbs.T4 Endolysin!}
-
CCATAGAATTCatgAGATCTggaggaaaaacatatgaatatatttgaaatgttacg
+
CCATAGAATTCatgAGATCTatacttaggaggtattatgaatatatttgaaatgttac
bx16      Reverse biobricking of {rbs.T4 Endolysin!}
bx16      Reverse biobricking of {rbs.T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtg
cgttaGGATCCtagatttttatacgcgtcccaagtg

Revision as of 00:00, 5 June 2008

Sixpi 22:25, 4 June 2008 (UTC)

PCR bx1/bx2 on Bjh1301		(688 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256		(EcoRI/BamHI)
Product is pBca1256-K112000
----------------------------------------
bx1		Forward Biobricking of {T4 Holin!}
CCATAGAATTCatgAGATCTatggcagcacctagaatatcat
bx2		Reverse Biobricking of {T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct

PCR bx3/bx2 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256		(EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3		Forward biobricking of {<T4 Holin!}
CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg
bx2		Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattc

PCR bx1/bx4 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256		(EcoRI/BamHI)
Product is pBca1256-K112002
----------------------------------------
bx1		Forward biobricking of {T4 Holin>}
CCATAGAATTCatgAGATCTatggcagcacctagaatatcat
bx4		Reverse biobricking of {T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc

PCR bx3/bx4 on Bjh1301		(682 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256		(EcoRI/BamHI)
Product is pBca1256-K112003
----------------------------------------
bx3		Forward Biobricking of {<T4 Holin>}
CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg
bx4		Reverse Biobricking of {<T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc

PCR bx3/bx4 on Bjh1301		(692 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256		(EcoRI/BamHI)
Product is pBca1256-K112004
----------------------------------------
bx5		Forward Biobricking of {a~T4 Holin!}
CCATAGAATTCatgAGATCTgtctatggcagcacctagaat
bx2		Reverse Biobricking of {a~T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct

PCR bx6/bx2 on Bjh1301		(704 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256		(EcoRI/BamHI)
Product is pBca1256-K112005
----------------------------------------
bx6		Forward Biobricking of {rbs.T4 Holin!}
CCATAGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc
bx2		Reverse Biobricking of {rbs.T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct

PCR bx7/bx8 on Bjh1303		(325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256		(EcoRI/BamHI)
Product is pBca1256-K112006
----------------------------------------
bx7		Forward biobricking of {T4 Antiholin!}
CCATAGAATTCatgAGATCTatggccttaaaagcaacagcac
bx8		Reverse biobricking of {T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg

PCR bx9/bx8 on Bjh1303		(322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256		(EcoRI/BamHI)
Product is pBca1256-K112007
----------------------------------------
bx9		Forward biobricking of {<T4 Antiholin!}
CCATAGAATTCatgAGATCTgccttaaaagcaacagcactt
bx8		Reverse biobricking of {<T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg

PCR bx7/bx10 on Bjh1303		(322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256		(EcoRI/BamHI)
Product is pBca1256-K112008
----------------------------------------
bx7		Forward biobricking of {T4 Antiholin>}
CCATAGAATTCatgAGATCTatggccttaaaagcaacagcac
bx10		Reverse biobricking of {T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc

PCR bx9/bx10 on Bjh1303		(319 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256		(EcoRI/BamHI)
Product is pBca1256-K112009
----------------------------------------
bx9		Forward biobricking of {<T4 Antiholin>}
CCATAGAATTCatgAGATCTgccttaaaagcaacagcactt
bx10		Reverse biobricking of {<T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc

PCR bx9/bx10 on Bjh1303		(329 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112010
----------------------------------------
bx11		Forward biobricking of {a~T4 Antiholin!}
CCATAGAATTCatgAGATCTctttatggccttaaaagcaacagc
bx8		Reverse biobricking of {a~T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg

PCR bx12/bx8 on Bjh1303		(339 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256		(EcoRI/BamHI)
Product is pBca1256-K112011
----------------------------------------
bx12	Forward biobricking of {rbs.T4 Antiholin!}
CCATAGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc
bx8		Reverse biobricking of {rbs.T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg

PCR bx13/bx14 on Bjh1302		(526 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112012
----------------------------------------
bx13      Forward biobricking of {T4 Endolysin!}
CCATAGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx14      Reverse biobricking of {T4 Endolysin!}
cgttaGGATCCttatagatttttatacgcgtccc

PCR bx15/bx14 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112013
----------------------------------------
bx15      Forward biobricking of {<T4 Endolysin!}
CCATAGAATTCatgAGATCTaatatatttgaaatgttacgtatag
bx14      Reverse biobricking of {<T4 Endolysin!}
cgttaGGATCCttatagatttttatacgcgtccc

PCR bx13/bx16 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112014
----------------------------------------
bx13      Forward biobricking of {T4 Endolysin>}
CCATAGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx16      Reverse biobricking of {T4 Endolysin>}
cgttaGGATCCtagatttttatacgcgtcccaagtg

PCR bx15/bx16 on Bjh1302		(520 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112015
----------------------------------------
bx15      Forward biobricking of {<T4 Endolysin>}
CCATAGAATTCatgAGATCTaatatatttgaaatgttacgtatag
bx16      Reverse biobricking of {<T4 Endolysin>}
cgttaGGATCCtagatttttatacgcgtcccaagtg

PCR bx17/bx16 on Bjh1302		(530 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112016
----------------------------------------
bx17      Forward biobricking of {a~T4 Endolysin!}
CCATAGAATTCatgAGATCTtattatgaatatatttgaaatg
bx16      Reverse biobricking of {a~T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtg

PCR bx18/bx16 on Bjh1302		(542 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112017
----------------------------------------
bx18      Forward biobricking of {rbs.T4 Endolysin!}
CCATAGAATTCatgAGATCTatacttaggaggtattatgaatatatttgaaatgttac
bx16      Reverse biobricking of {rbs.T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtg



Bing Xia

Retrieved from "http://2008.igem.org/Template:Team:UC_Berkeley/Notebook/BX_construction"