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Team:NTU-Singapore/Notebook/2 September 2008
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(Difference between revisions)
(New page: =Tuesday 2 September= *Morning: Lu Chao: minipreps for 6 samples from Monday and prepared 1% gel. *Hung: digestional gel check.<br/>Results: **Among 2 samples of LacI-RBS-E7-Term, 1 sample...) |
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*Morning: Lu Chao: minipreps for 6 samples from Monday and prepared 1% gel. | *Morning: Lu Chao: minipreps for 6 samples from Monday and prepared 1% gel. | ||
*Hung: digestional gel check.<br/>Results: | *Hung: digestional gel check.<br/>Results: | ||
| - | **Among 2 samples of LacI-RBS-E7-Term, 1 sample showed correct bands!! 2.5 kb insert (total size of LacI+RBS+E7+Term) and 2kb vector (standard vector). The other sample is uncut LacI-RBS plasmid. | + | **Among 2 samples of LacI-RBS-E7-Term, 1 sample showed correct bands!! 2.5 kb insert (total size of LacI+RBS+E7+Term) and 2kb vector (standard vector). The other sample is uncut LacI-RBS plasmid. The E7-Term was run together for comparison purpose. |
**The other samples (for minipreps) were correct. | **The other samples (for minipreps) were correct. | ||
[[Image:NTU_gelcheck_0209.JPG|500px|center|thumb|Gel check 2 September.]] | [[Image:NTU_gelcheck_0209.JPG|500px|center|thumb|Gel check 2 September.]] | ||
YES!! So our production system is finally completed!! | YES!! So our production system is finally completed!! | ||
Revision as of 12:14, 2 September 2008
Tuesday 2 September
- Morning: Lu Chao: minipreps for 6 samples from Monday and prepared 1% gel.
- Hung: digestional gel check.
Results:- Among 2 samples of LacI-RBS-E7-Term, 1 sample showed correct bands!! 2.5 kb insert (total size of LacI+RBS+E7+Term) and 2kb vector (standard vector). The other sample is uncut LacI-RBS plasmid. The E7-Term was run together for comparison purpose.
- The other samples (for minipreps) were correct.
YES!! So our production system is finally completed!!
