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Team:KULeuven/2 September 2008
From 2008.igem.org
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* We tried to construct our parts K145150 (hybrid promoter) and K145014 (T7 polymerase with UmuD tag) using PCR. Both of them failed, but K145150 gave two bands. This is probably because we didn't purify it at first. We will do that tomorrow. | * We tried to construct our parts K145150 (hybrid promoter) and K145014 (T7 polymerase with UmuD tag) using PCR. Both of them failed, but K145150 gave two bands. This is probably because we didn't purify it at first. We will do that tomorrow. | ||
* We made electrocompetent cells of the JM109 stem. | * We made electrocompetent cells of the JM109 stem. | ||
| - | * We did some more digests: | + | * We did some more digests: <div style="margin-left:10px;"> cut with ''Spe''I and ''Eco''RI -> R0040, B0014, R0053 and R1052 (R1052 failed). </div> <div style="margin-left:10px;"> cut with ''Xba''I -> K145151+B0015, C0040+B0015, C0060+B0015, C0012+B0015 and K145015+B0015. </div> |
| - | <div style="margin-left: | + | |
| - | <div style="margin-left: | + | |
* We started a few ligations: R0040+B0033, R0053+P0152, R0040+J23066, B0014+B0033 and R0040+E0240. | * We started a few ligations: R0040+B0033, R0053+P0152, R0040+J23066, B0014+B0033 and R0040+E0240. | ||
* We also tested the ligations of 31 August using PCR. Most of them were OK. The ligations that succeeded so far can be found on the schemes below. | * We also tested the ligations of 31 August using PCR. Most of them were OK. The ligations that succeeded so far can be found on the schemes below. | ||
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<div style="margin-left:100px;"> Current Status (Final Scheme): </div> | <div style="margin-left:100px;"> Current Status (Final Scheme): </div> | ||
[[Image:2sept final.PNG|center]] | [[Image:2sept final.PNG|center]] | ||
Revision as of 20:07, 5 September 2008
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Contents |
Lab Work
Wet Lab
- We tried to construct our parts K145150 (hybrid promoter) and K145014 (T7 polymerase with UmuD tag) using PCR. Both of them failed, but K145150 gave two bands. This is probably because we didn't purify it at first. We will do that tomorrow.
- We made electrocompetent cells of the JM109 stem.
- We did some more digests: cut with SpeI and EcoRI -> R0040, B0014, R0053 and R1052 (R1052 failed).cut with XbaI -> K145151+B0015, C0040+B0015, C0060+B0015, C0012+B0015 and K145015+B0015.
- We started a few ligations: R0040+B0033, R0053+P0152, R0040+J23066, B0014+B0033 and R0040+E0240.
- We also tested the ligations of 31 August using PCR. Most of them were OK. The ligations that succeeded so far can be found on the schemes below.
Current Status (Final Scheme):
Current Status (Parallel Scheme):
Dry Lab
Ethics...
Modeling
Latex-tool... Multi-cell... Diffusion... Updating wiki...
Wiki
Remarks
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