Template:Team:UC Berkeley/Notebook/Molly construction

From 2008.igem.org

(Difference between revisions)
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mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtgacttcgccttcttcc
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtgacttcgccttcttcc
 +
</pre>
 +
 +
<pre>
 +
Construction of {<xis!} Biobrick Part K112201
 +
PCR mea003/mea002 on pAH57 (227 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112201
 +
----------------------------------------
 +
mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg
 +
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtgacttcgccttcttcc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {xis>} Biobrick Part K112202
 +
PCR mea001/mea004 on pAH57 (227 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112202
 +
----------------------------------------
 +
mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag
 +
mea004 Reverse Biobricking of {xis>} cgttaGGATCCtgacttcgccttcttcccatttc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {<xis>} Biobrick Part K112203
 +
PCR mea003/mea004 on pAH57 (224 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112203
 +
----------------------------------------
 +
mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg
 +
mea004 Reverse Biobricking of {xis>} cgttaGGATCCtgacttcgccttcttcccatttc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {a~xis!} Biobrick Part K112204
 +
PCR mea017/mea002 on pAH57 (234 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112204
 +
----------------------------------------
 +
mea017 Forward Biobricking of {a~xis!}ccataGAATTCatgAGATCTtgcgatgtacttgacacttcagg
 +
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtgacttcgccttcttcc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {rbs.xis!} Biobrick Part K112205
 +
PCR mea018/mea002 on pAH57 (249 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112205
 +
----------------------------------------
 +
mea018 Forward Biobricking of {rbs.xis!}ccataGAATTCatgAGATCTtaattgcggagactttgcgatg
 +
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtgacttcgccttcttcc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {int!} Biobrick Part K112207
 +
PCR mea005/mea006 on pAH57 (1102 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112207
 +
----------------------------------------
 +
mea005 Forward Biobricking of {int!} ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
 +
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {<int!} Biobrick Part K112208
 +
PCR mea007/mea006 on pAH57 (1099 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112208
 +
----------------------------------------
 +
mea007 Forward Biobricking of {<int!} ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
 +
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {int>} Biobrick Part K112209
 +
PCR mea005/mea008 on pAH57 (1099 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112209
 +
----------------------------------------
 +
mea005 Forward Biobricking of {int!} ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
 +
mea008 Reverse Biobricking of {int>} cgttaGGATCCtttgatttcaattttgtcccactccc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {<int>} Biobrick Part K112210
 +
PCR mea007/mea008 on pAH57 (1096 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112210
 +
----------------------------------------
 +
mea007 Forward Biobricking of {<int!} ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
 +
mea008 Reverse Biobricking of {int>} cgttaGGATCCtttgatttcaattttgtcccactccc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {a~int!} Biobrick Part K112211
 +
PCR mea019/mea006 on pAH57 (1106 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112211
 +
----------------------------------------
 +
mea019 Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag
 +
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {rbs.int!} Biobrick Part K112212
 +
PCR mea020/mea006 on pAH57 (1120 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112212
 +
----------------------------------------
 +
mea020 Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg
 +
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {ihfA!} Biobrick Part K112213
 +
PCR mea009/mea010 on MG1655 (331 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112213
 +
----------------------------------------
 +
mea009 Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
 +
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {<ihfA!} Biobrick Part K112214
 +
PCR mea011/mea010 on MG1655 (328 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112214
 +
----------------------------------------
 +
mea011 Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
 +
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {ihfA>} Biobrick Part K112215
 +
PCR mea009/mea012 on MG1655 (328 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112215
 +
----------------------------------------
 +
mea009 Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
 +
mea012 Reverse Biobricking of {ihfA>} cgttaGGATCCctcgtctttgggcgaagcg
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {<ihfA>} Biobrick Part K112216
 +
PCR mea011/mea012 on MG1655 (325 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112216
 +
----------------------------------------
 +
mea011 Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
 +
mea012 Reverse Biobricking of {ihfA>} cgttaGGATCCctcgtctttgggcgaagcg
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {a~ihfA!} Biobrick Part K112217
 +
PCR mea021/mea010 on MG1655 (335 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112217
 +
----------------------------------------
 +
mea021 Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc
 +
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {rbs.ihfA!} Biobrick Part K112218
 +
PCR mea022/mea010 on MG1655 (350 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112218
 +
----------------------------------------
 +
mea022 Forward Biobricking of {rbs.ihfA!}ccataGAATTCatgAGATCTtcattgagggattgaacctatgg
 +
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {ihfB!} Biobrick Part K112219
 +
PCR mea013/mea014 on MG1655 (316 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112219
 +
----------------------------------------
 +
mea013 Forward Biobricking of {ihfB!}ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagac
 +
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {<ihfB!} Biobrick Part K112220
 +
PCR mea015/mea014 on MG1655 (313 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112220
 +
----------------------------------------
 +
mea015 Forward Biobricking of {<ihfB!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
 +
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {ihfB>} Biobrick Part K112221
 +
PCR mea013/mea016 on MG1655 (313 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112221
 +
----------------------------------------
 +
mea013 Forward Biobricking of {ihfB!}ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagac
 +
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {<ihfB>} Biobrick Part K112222
 +
PCR mea015/mea016 on MG1655 (310 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112222
 +
----------------------------------------
 +
mea015 Forward Biobricking of {<ihfB!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc
 +
</pre>
 +
 +
<pre>
 +
Construction of {a~ihfB!} Biobrick Part K112223
 +
PCR mea023/mea014 on MG1655 (310 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112223
 +
----------------------------------------
 +
mea023 Forward Biobricking of {a~ihfB!} ccataGAATTCatgAGATCTaatcatgaccaagtcagaattgatag
 +
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
 +
</pre>
 +
 +
 +
<pre>
 +
Construction of {rbs.ihfB!} Biobrick Part K112224
 +
PCR mea024/mea014 on MG1655 (337 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112224
 +
----------------------------------------
 +
mea024 Forward Biobricking of {rbs.ihfB!} ccataGAATTCatgAGATCTctttaaggaaccggaggaatcatg
 +
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
</pre>
</pre>
[[Team:UC_Berkeley/Notebook/Molly_Allen|Molly's Notebook]]<br>
[[Team:UC_Berkeley/Notebook/Molly_Allen|Molly's Notebook]]<br>

Revision as of 06:38, 5 June 2008

Construction of {xis!} Biobrick Part K112200
PCR mea001/mea002 on pAH57		(230 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112200
----------------------------------------
mea001	Forward Biobricking of {xis!}	ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtgacttcgccttcttcc

Construction of {<xis!} Biobrick Part K112201
PCR mea003/mea002 on pAH57		(227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112201
----------------------------------------
mea003	Forward Biobricking of {<xis!}	ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtgacttcgccttcttcc



Construction of {xis>} Biobrick Part K112202
PCR mea001/mea004 on pAH57		(227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112202
----------------------------------------
mea001	Forward Biobricking of {xis!}	ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea004	Reverse Biobricking of {xis>}	cgttaGGATCCtgacttcgccttcttcccatttc



Construction of {<xis>} Biobrick Part K112203
PCR mea003/mea004 on pAH57		(224 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112203
----------------------------------------
mea003	Forward Biobricking of {<xis!}	ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea004	Reverse Biobricking of {xis>}	cgttaGGATCCtgacttcgccttcttcccatttc



Construction of {a~xis!} Biobrick Part K112204
PCR mea017/mea002 on pAH57		(234 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112204
----------------------------------------
mea017	Forward Biobricking of {a~xis!}ccataGAATTCatgAGATCTtgcgatgtacttgacacttcagg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtgacttcgccttcttcc



Construction of {rbs.xis!} Biobrick Part K112205
PCR mea018/mea002 on pAH57		(249 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112205
----------------------------------------
mea018	Forward Biobricking of {rbs.xis!}ccataGAATTCatgAGATCTtaattgcggagactttgcgatg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtgacttcgccttcttcc



Construction of {int!} Biobrick Part K112207
PCR mea005/mea006 on pAH57		(1102 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112207
----------------------------------------
mea005	Forward Biobricking of {int!}	ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactc



Construction of {<int!} Biobrick Part K112208
PCR mea007/mea006 on pAH57		(1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112208
----------------------------------------
mea007	Forward Biobricking of {<int!}	ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactc



Construction of {int>} Biobrick Part K112209
PCR mea005/mea008 on pAH57		(1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112209
----------------------------------------
mea005	Forward Biobricking of {int!}	ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea008	Reverse Biobricking of {int>}	cgttaGGATCCtttgatttcaattttgtcccactccc



Construction of {<int>} Biobrick Part K112210
PCR mea007/mea008 on pAH57		(1096 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112210
----------------------------------------
mea007	Forward Biobricking of {<int!}	ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea008	Reverse Biobricking of {int>}	cgttaGGATCCtttgatttcaattttgtcccactccc



Construction of {a~int!} Biobrick Part K112211
PCR mea019/mea006 on pAH57		(1106 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112211
----------------------------------------
mea019	Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactc



Construction of {rbs.int!} Biobrick Part K112212
PCR mea020/mea006 on pAH57		(1120 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112212
----------------------------------------
mea020	Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactc



Construction of {ihfA!} Biobrick Part K112213
PCR mea009/mea010 on MG1655		(331 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112213
----------------------------------------
mea009	Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagc



Construction of {<ihfA!} Biobrick Part K112214
PCR mea011/mea010 on MG1655		(328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112214
----------------------------------------
mea011	Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagc



Construction of {ihfA>} Biobrick Part K112215
PCR mea009/mea012 on MG1655		(328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112215
----------------------------------------
mea009	Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea012	Reverse Biobricking of {ihfA>}	cgttaGGATCCctcgtctttgggcgaagcg



Construction of {<ihfA>} Biobrick Part K112216
PCR mea011/mea012 on MG1655		(325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112216
----------------------------------------
mea011	Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea012	Reverse Biobricking of {ihfA>}	cgttaGGATCCctcgtctttgggcgaagcg



Construction of {a~ihfA!} Biobrick Part K112217
PCR mea021/mea010 on MG1655		(335 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112217
----------------------------------------
mea021	Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagc



Construction of {rbs.ihfA!} Biobrick Part K112218
PCR mea022/mea010 on MG1655		(350 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112218
----------------------------------------
mea022	Forward Biobricking of {rbs.ihfA!}ccataGAATTCatgAGATCTtcattgagggattgaacctatgg
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagc



Construction of {ihfB!} Biobrick Part K112219
PCR mea013/mea014 on MG1655		(316 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112219
----------------------------------------
mea013	Forward Biobricking of {ihfB!}ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagac
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg



Construction of {<ihfB!} Biobrick Part K112220
PCR mea015/mea014 on MG1655		(313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112220
----------------------------------------
mea015	Forward Biobricking of {<ihfB!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg



Construction of {ihfB>} Biobrick Part K112221
PCR mea013/mea016 on MG1655		(313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112221
----------------------------------------
mea013	Forward Biobricking of {ihfB!}ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagac
mea016	Reverse Biobricking of {ihfB>}	cgttaGGATCCaccgtaaatattggcgcgatc



Construction of {<ihfB>} Biobrick Part K112222
PCR mea015/mea016 on MG1655		(310 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112222
----------------------------------------
mea015	Forward Biobricking of {<ihfB!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc mea016	Reverse Biobricking of {ihfB>}	cgttaGGATCCaccgtaaatattggcgcgatc

Construction of {a~ihfB!} Biobrick Part K112223
PCR mea023/mea014 on MG1655		(310 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112223
----------------------------------------
mea023	Forward Biobricking of {a~ihfB!}	ccataGAATTCatgAGATCTaatcatgaccaagtcagaattgatag
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg



Construction of {rbs.ihfB!} Biobrick Part K112224
PCR mea024/mea014 on MG1655		(337 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112224
----------------------------------------
mea024	Forward Biobricking of {rbs.ihfB!}	ccataGAATTCatgAGATCTctttaaggaaccggaggaatcatg
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg

Molly's Notebook

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