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| - | =Construction of rbs-FlhDC-mRFP-LVA-tripart(L172)= | + | =Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)= |
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| | '''Ligation L172''' | | '''Ligation L172''' |
Revision as of 20:19, 3 September 2008
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← Yesterday ↓ Calendar ↑Tomorrow →
Construction of rbs-TetR-mRFP-LVA-tripart
Ligation L173
Samples used
| Name
| Digestion number
| Digested by
| DNA concentration
|
| rbs-TetR
| D112
| EcoRI & SpeI (insert)
| ~20 µg/mL
|
| mRFP-LVA-tripart
| D187
| EcoRI & XbaI (vector)
| 16 µg/mL
|
Reaction mixtures
|
| Control
| Sample
|
| 3:1 (insert:vector) ratio
| 4:1 (insert:vector) ratio
|
| D112 (insert)
| 0 µL
| 1,4 µL
| 1,9 µL
|
| D187 (vector)
| 2,5 µL
| 2,5 µL
| 2,5 µL
|
| T4 DNA ligase 10X buffer
| 2 µL
| 2 µL
| 2 µL
|
| T4 DNA ligase
| 1 µL
| 1 µL
| 1 µL
|
| water
| 14,5 µL
| 13,1 µL
| 12,6 µL
|
- Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction).
Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)
Ligation L172
| Name
| Digestion number
| Digested by
| DNA concentration
|
| rbs-FlhDC
| D186
| EcoRI & SpeI (insert)
| ~24 µg/mL
|
| mRFP-LVA-tripart
| D187
| EcoRI & XbaI (vector)
| 16 µg/mL
|
Reaction mixtures
|
| Control
| Sample
|
| D186 (insert)
| 0 µL
| 1 µL
|
| D187 (vector)
| 2,5 µL
| 2,5 µL
|
| T4 DNA ligase 10X buffer
| 2 µL
| 2 µL
|
| T4 DNA ligase
| 1 µL
| 1 µL
|
| water
| 14,5 µL
| 13,5 µL
|
- Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction).
|
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