Team:Paris/Modeling/f2

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[[Image:f2.jpg|thumb]] According to previous considerations on pBad, we have
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[[Image:f2.jpg|thumb]]
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<center>[[Image:f2expr.jpg]]</center>
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The experience would give us
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so, with the complexation between Arabinose and AraC, and after reducing the equation
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[[Image:f2expr.jpg|center]]
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<center>[[Image:f2exprRedu.jpg]]</center>
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Thus, at steady-state and in the exponential phase of growth :
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[[Image:Exprpbad.jpg|center]]
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Revision as of 23:37, 29 September 2008

F2.jpg

The experience would give us

F2expr.jpg

Thus, at steady-state and in the exponential phase of growth :

Exprpbad.jpg
param signification unit value
[expr(pBad)] expression rate of
pBad with RBS E0032 		nM.min-1 see "findparam"
need for 20 measures
γGFP dilution-degradation rate
of GFP(mut3b) 		min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 measures
(fluorescence) value of the observed fluorescence au need for 20 measures
conversion conversion ration between
fluorescence and concentration 		nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations 		unit value
βbad production rate of pBad with RBS E0032
not in the system 		nM.min-1
(Kbad/[AraCtot]) activation constant of pBad
not in the system 		nM
nbad complexation order of pBad
not in the system 		no dimension
Kara complexation constant Arabinose-AraC
not in the system 		nM
nara complexation order Arabinose-AraC
not in the system 		no dimension
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