Team:Paris/Modeling/f6

From 2008.igem.org

(Difference between revisions)
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[[Image:f6DCA.png|thumb]] At the steady-state, we have
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[[Image:f6DCA.png|thumb]]
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<center>[[Image:FlhDCeq.jpg]]</center>
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and
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<center>[[Image:FliAeq.jpg]]</center>
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so the expression
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<center>[[Image:f6expr.jpg]]</center>
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gives
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<center>[[Image:f6FlhDC.jpg]]</center>
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and
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<center>[[Image:f6FliA.jpg]]</center>
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and for ''calculated values of the TF'',
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<center>[[Image:f6FlhDCCalc.jpg]]</center>
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and
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<center>[[Image:f6FliACalc.jpg]]</center>
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<br><br>
<br><br>
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|''conversion''
|''conversion''
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|conversion ration between <br> fluorescence and concentration
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|conversion ratio between <br> fluorescence and concentration
|nM.au<sup>-1</sup>
|nM.au<sup>-1</sup>
|(1/79.429)
|(1/79.429)
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Then, if we have time, we want to verify the expected relation
Then, if we have time, we want to verify the expected relation
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<center>[[Image:SumpFlgA.jpg]]</center>
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[[Image:SumpFlgA.jpg|center]]

Revision as of 23:48, 29 September 2008

F6DCA.png



param signification unit value
[expr(pFlgA)] expression rate of
pFlgA with RBS E0032
nM.min-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ratio between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β55 production rate of FlhDC-pFlgA with RBS E0032
β55
nM.min-1
(K49/{coefflhDC}) activation constant of FlhDC-pFlgA
K49
nM
n49 complexation order of FlhDC-pFlgA
n49
no dimension
β56 production rate of FliA-pFlgA with RBS E0032
β56
nM.min-1
(K50/{coeffliA}) activation constant of FliA-pFlgA
K50
nM
n50 complexation order of FliA-pFlgA
n50
no dimension



Then, if we have time, we want to verify the expected relation

SumpFlgA.jpg