Template:Team:UC Berkeley/Notebook/Molly construction

From 2008.igem.org

(Difference between revisions)

Revision as of 01:33, 6 June 2008

Construction of {xis!} Biobrick Part K112200
PCR mea001/mea002 on pAH57		(230 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112200
----------------------------------------
mea001	Forward Biobricking of {xis!}	ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc

Construction of {<xis!} Biobrick Part K112201
PCR mea003/mea002 on pAH57		(227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112201
----------------------------------------
mea003	Forward Biobricking of {<xis!}	ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttccc

Construction of {xis>} Biobrick Part K112202
PCR mea001/mea004 on pAH57		(227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112202
----------------------------------------
mea001	Forward Biobricking of {xis!}	ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea004	Reverse Biobricking of {xis>}	cgttaGGATCCtgacttcgccttcttcccatttc

Construction of {<xis>} Biobrick Part K112203
PCR mea003/mea004 on pAH57		(224 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112203
----------------------------------------
mea003	Forward Biobricking of {<xis!}	ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea004	Reverse Biobricking of {xis>}	cgttaGGATCCtgacttcgccttcttcccatttc

Construction of {a~xis!} Biobrick Part K112204
PCR mea017/mea002 on pAH57		(234 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112204
----------------------------------------
mea017	Forward Biobricking of {a~xis!}ccataGAATTCatgAGATCTtgcgatgtacttgacacttcagg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtcatgacttcgccttcttcc

Construction of {rbs.xis!} Biobrick Part K112205
PCR mea018/mea002 on pAH57		(249 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112205
----------------------------------------
mea018	Forward Biobricking of {rbs.xis!}ccataGAATTCatgAGATCTtaattgcggagactttgcgatg
mea002	Reverse Biobricking of {xis!}	cgttaGGATCCtgacttcgccttcttcc

Construction of {int!} Biobrick Part K112207
PCR mea005/mea006 on pAH57		(1102 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112207
----------------------------------------
mea005	Forward Biobricking of {int!}	ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactc

Construction of {<int!} Biobrick Part K112208
PCR mea007/mea006 on pAH57		(1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112208
----------------------------------------
mea007	Forward Biobricking of {<int!}	ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactc

Construction of {int>} Biobrick Part K112209
PCR mea005/mea008 on pAH57		(1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112209
----------------------------------------
mea005	Forward Biobricking of {int!}	ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea008	Reverse Biobricking of {int>}	cgttaGGATCCtttgatttcaattttgtcccactccc

Construction of {<int>} Biobrick Part K112210
PCR mea007/mea008 on pAH57		(1096 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112210
----------------------------------------
mea007	Forward Biobricking of {<int!}	ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea008	Reverse Biobricking of {int>}	cgttaGGATCCtttgatttcaattttgtcccactccc

Construction of {a~int!} Biobrick Part K112211
PCR mea019/mea006 on pAH57		(1106 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112211
----------------------------------------
mea019	Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactc

Construction of {rbs.int!} Biobrick Part K112212
PCR mea020/mea006 on pAH57		(1120 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112212
----------------------------------------
mea020	Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg
mea006	Reverse Biobricking of {int!}	cgttaGGATCCttatttgatttcaattttgtcccactc

Construction of {ihfA!} Biobrick Part K112213
PCR mea009/mea010 on MG1655		(331 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112213
----------------------------------------
mea009	Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagc

Construction of {<ihfA!} Biobrick Part K112214
PCR mea011/mea010 on MG1655		(328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112214
----------------------------------------
mea011	Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagc

Construction of {ihfA>} Biobrick Part K112215
PCR mea009/mea012 on MG1655		(328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112215
----------------------------------------
mea009	Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea012	Reverse Biobricking of {ihfA>}	cgttaGGATCCctcgtctttgggcgaagcg

Construction of {<ihfA>} Biobrick Part K112216
PCR mea011/mea012 on MG1655		(325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112216
----------------------------------------
mea011	Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea012	Reverse Biobricking of {ihfA>}	cgttaGGATCCctcgtctttgggcgaagcg

Construction of {a~ihfA!} Biobrick Part K112217
PCR mea021/mea010 on MG1655		(335 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112217
----------------------------------------
mea021	Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagc

Construction of {rbs.ihfA!} Biobrick Part K112218
PCR mea022/mea010 on MG1655		(350 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112218
----------------------------------------
mea022	Forward Biobricking of {rbs.ihfA!}ccataGAATTCatgAGATCTtcattgagggattgaacctatgg
mea010	Reverse Biobricking of {ihfA!}	cgttaGGATCCttactcgtctttgggcgaagc

Construction of {ihfB!} Biobrick Part K112219
PCR mea013/mea014 on MG1655		(316 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112219
----------------------------------------
mea013	Forward Biobricking of {ihfB!}ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagac
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg

Construction of {<ihfB!} Biobrick Part K112220
PCR mea015/mea014 on MG1655		(313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112220
----------------------------------------
mea015	Forward Biobricking of {<ihfB!}ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagac
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg

Construction of {ihfB>} Biobrick Part K112221
PCR mea013/mea016 on MG1655		(313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112221
----------------------------------------
mea013	Forward Biobricking of {ihfB!}ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagac
mea016	Reverse Biobricking of {ihfB>}	cgttaGGATCCaccgtaaatattggcgcgatc

Construction of {<ihfB>} Biobrick Part K112222
PCR mea015/mea016 on MG1655		(310 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112222
----------------------------------------
mea015	Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagac
mea016	Reverse Biobricking of {ihfB>}	cgttaGGATCCaccgtaaatattggcgcgatc

Construction of {a~ihfB!} Biobrick Part K112223
PCR mea023/mea014 on MG1655		(310 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112223
----------------------------------------
mea023	Forward Biobricking of {a~ihfB!}ccataGAATTCatgAGATCTaatcatgaccaagtcagaattgatag
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg

Construction of {rbs.ihfB!} Biobrick Part K112224
PCR mea024/mea014 on MG1655		(337 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112224
----------------------------------------
mea024	Forward Biobricking of {rbs.ihfB!}ccataGAATTCatgAGATCTctttaaggaaccggaggaatcatg
mea014	Reverse Biobricking of {ihfB!}	cgttaGGATCCttaaccgtaaatattggcgcgatcg