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Team:Guelph/Modeling
From 2008.igem.org
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| - | We plan to do wet lab modelling. By tagging our microbes with simple GFP constructs and | + | We plan to do wet lab modelling. By tagging our microbes with simple GFP constructs and counting colony forming units per set sample fresh weight, we hope to be able to observe microbial survival with our construct and predict how much beta carotene or RNAi might be produced and released. |
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| + | In corn, we are doing this using a corn endophyte called Klebsiella pneumonii. Plants were either injected with 5 ul of bacterial suspension or dipped in it at an early stage. Two weeks later (a month for the dipped plants) 500 mg of tissue were harvested, ground in sterilized mortars, resuspended in 500 ul of sodium phosphate buffer, and 50 ul of the dilution was spread on half of a kanamycin LB plate. The results are depicted graphically below. | ||
[[Image:KP342_GFP_cornassay.jpg|700px]] | [[Image:KP342_GFP_cornassay.jpg|700px]] | ||
Revision as of 00:10, 9 September 2008
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Making projections based on measurements on endosymbiont and endophyte numbers within a functioning biological system using GFP
We plan to do wet lab modelling. By tagging our microbes with simple GFP constructs and counting colony forming units per set sample fresh weight, we hope to be able to observe microbial survival with our construct and predict how much beta carotene or RNAi might be produced and released.
In corn, we are doing this using a corn endophyte called Klebsiella pneumonii. Plants were either injected with 5 ul of bacterial suspension or dipped in it at an early stage. Two weeks later (a month for the dipped plants) 500 mg of tissue were harvested, ground in sterilized mortars, resuspended in 500 ul of sodium phosphate buffer, and 50 ul of the dilution was spread on half of a kanamycin LB plate. The results are depicted graphically below.
Note
If you choose to include a Modeling page, please write about your modeling adventures here. This is not necessary but it may be a nice list to include.
