Template:Team:UC Berkeley/Notebook/BX construction

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Revision as of 21:05, 9 June 2008

PCR bx1/bx2 on Bjh1301		(688 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112000
----------------------------------------
bx1		Forward Biobricking of {T4 Holin!}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx2		Reverse Biobricking of {T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

PCR bx3/bx2 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3		Forward biobricking of {<T4 Holin!}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx2		Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

PCR bx1/bx4 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112002
----------------------------------------
bx1		Forward biobricking of {T4 Holin>}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx4		Reverse biobricking of {T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc

PCR bx3/bx4 on Bjh1301		(682 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112003
----------------------------------------
bx3		Forward Biobricking of {<T4 Holin>}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx4		Reverse Biobricking of {<T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc

PCR bx5/bx2 on Bjh1301		(692 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112004
----------------------------------------
bx5		Forward Biobricking of {a~T4 Holin!}
ccagtGAATTCatgAGATCTgtctatggcagcacctagaatatc
bx2		Reverse Biobricking of {a~T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

PCR bx6/bx2 on Bjh1301		(704 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112005
----------------------------------------
bx6		Forward Biobricking of {rbs.T4 Holin!}
ccagtGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc
bx2		Reverse Biobricking of {rbs.T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

PCR bx7/bx8 on Bjh1303		(325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112006
----------------------------------------
bx7		Forward biobricking of {T4 Antiholin!}
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
bx8		Reverse biobricking of {T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

PCR bx9/bx8 on Bjh1303		(322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112007
----------------------------------------
bx9		Forward biobricking of {<T4 Antiholin!}
ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
bx8		Reverse biobricking of {<T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

PCR bx7/bx10 on Bjh1303		(322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112008
----------------------------------------
bx7		Forward biobricking of {T4 Antiholin>}
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
bx10	Reverse biobricking of {T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc

PCR bx9/bx10 on Bjh1303		(319 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112009
----------------------------------------
bx9		Forward biobricking of {<T4 Antiholin>}
ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
bx10	Reverse biobricking of {<T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc

PCR bx11/bx8 on Bjh1303		(329 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112010
----------------------------------------
bx11	Forward biobricking of {a~T4 Antiholin!}
ccagtGAATTCatgAGATCTctttatggccttaaaagcaacagc
bx8		Reverse biobricking of {a~T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

PCR bx12/bx8 on Bjh1303		(339 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112011
----------------------------------------
bx12	Forward biobricking of {rbs.T4 Antiholin!}
ccagtGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc
bx8		Reverse biobricking of {rbs.T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

PCR bx13/bx14 on Bjh1302		(526 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112012
----------------------------------------
bx13      Forward biobricking of {T4 Endolysin!}
ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx14      Reverse biobricking of {T4 Endolysin!}
cgttaGGATCCttatagatttttatacgcgtcccaagtgcc

PCR bx15/bx14 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112013
----------------------------------------
bx15      Forward biobricking of {<T4 Endolysin!}
ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg
bx14      Reverse biobricking of {<T4 Endolysin!}
cgttaGGATCCttatagatttttatacgcgtcccaagtgcc

PCR bx13/bx16 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112014
----------------------------------------
bx13      Forward biobricking of {T4 Endolysin>}
ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx16      Reverse biobricking of {T4 Endolysin>}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

PCR bx15/bx16 on Bjh1302		(520 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112015
----------------------------------------
bx15      Forward biobricking of {<T4 Endolysin>}
ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg
bx16      Reverse biobricking of {<T4 Endolysin>}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

PCR bx17/bx16 on Bjh1302		(530 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112016
----------------------------------------
bx17      Forward biobricking of {a~T4 Endolysin!}
ccagtGAATTCatgAGATCTtattatgaatatatttgaaatgttacgtatagatg
bx16      Reverse biobricking of {a~T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

PCR bx18/bx16 on Bjh1302		(542 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112017
----------------------------------------
bx18      Forward biobricking of {rbs.T4 Endolysin!}
ccagtGAATTCatgAGATCTatacttaggaggtattatgaatatatttgaaatgttac
bx16      Reverse biobricking of {rbs.T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

Bing Xia

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