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Team:Warsaw/Calendar-Main/16 May 2008
From 2008.igem.org
(Difference between revisions)
(New page: {{WarNotebook}} <!-- do not edit above me! --> 1. PCR - AID for translation fusion Primers: AIDlNrH AIDpLinB Template DNA: pTrc99A-AID Annealing temperature: 55 °C Annealing time: 6...) |
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Primers: | Primers: | ||
| - | AIDlNrH | + | <html> |
| - | AIDpLinB | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> |
| - | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDpLinB">AIDpLinB</a> | |
| + | <br> | ||
| + | </html> | ||
Template DNA: pTrc99A-AID | Template DNA: pTrc99A-AID | ||
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Primers: | Primers: | ||
| - | T7lLinkB | + | <html> |
| - | T7pXbSal | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7lLinkB">T7lLinkB</a> |
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a> | ||
| + | <br> | ||
| + | </html> | ||
Template DNA: E. coli Rosetta genomic DNA | Template DNA: E. coli Rosetta genomic DNA | ||
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20 cycles | 20 cycles | ||
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3. PCR - T7 RNA-polimerase for transcription fusion | 3. PCR - T7 RNA-polimerase for transcription fusion | ||
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Primers: | Primers: | ||
| - | T7lRBSHi | + | <html> |
| - | T7pXbSal | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7lRBSHi">T7lRBSHi</a> |
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a> | ||
| + | <br> | ||
| + | </html> | ||
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Template DNA: E. coli Rosetta genomic DNA | Template DNA: E. coli Rosetta genomic DNA | ||
Revision as of 13:41, 26 September 2008
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1. PCR - AID for translation fusion Primers:
AIDlNrH
AIDpLinB
Annealing temperature: 55 °C Annealing time: 60 sec 20 cycles
Primers: Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles 3. PCR - T7 RNA-polimerase for transcription fusion Primers:
Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles
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