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Team:University of Ottawa/5 June 2008
From 2008.igem.org
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==Today in the Lab== | ==Today in the Lab== | ||
'''Dan, Matt''' | '''Dan, Matt''' | ||
| + | :'''igestion''' | ||
::<li>We decided to use different enzymes - Bam HI + EcoRI for 8/7 and xhoI for 0/1. | ::<li>We decided to use different enzymes - Bam HI + EcoRI for 8/7 and xhoI for 0/1. | ||
| + | :'''Gel Results''' | ||
::<li>Gel was unsuccessful for 1A but results for other plasmids worked well. | ::<li>Gel was unsuccessful for 1A but results for other plasmids worked well. | ||
::<li>We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them. | ::<li>We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them. | ||
| - | ::<li>Colonies were then inoculated using LB + solution and left overnight. | + | ::<li>Colonies were then inoculated using LB + Amp solution and left overnight. |
| + | :'''Glycerol Stock''' | ||
| + | ::<li>Glycerol Stock was performed for plasmids 7/8 and 0. | ||
Revision as of 18:25, 6 June 2008
Today in the Lab
Dan, Matt
- igestion
- We decided to use different enzymes - Bam HI + EcoRI for 8/7 and xhoI for 0/1.
- Gel Results
- Gel was unsuccessful for 1A but results for other plasmids worked well.
- We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them.
- Colonies were then inoculated using LB + Amp solution and left overnight.
- Glycerol Stock
- Glycerol Stock was performed for plasmids 7/8 and 0.
