Team:University of Ottawa/5 June 2008
From 2008.igem.org
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:'''Gel Results''' | :'''Gel Results''' | ||
::<li>Gel was unsuccessful for 1A but results for other plasmids worked well. | ::<li>Gel was unsuccessful for 1A but results for other plasmids worked well. | ||
+ | :'''Miniprep''' | ||
::<li>We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them. | ::<li>We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them. | ||
::<li>Colonies were then inoculated using LB + Amp solution and left overnight. | ::<li>Colonies were then inoculated using LB + Amp solution and left overnight. | ||
:'''Glycerol Stock''' | :'''Glycerol Stock''' | ||
::<li>Glycerol Stock was performed for plasmids 7/8 and 0. | ::<li>Glycerol Stock was performed for plasmids 7/8 and 0. |
Revision as of 18:26, 6 June 2008
Today in the Lab
Dan, Matt
- Digestion
- We decided to use different enzymes - Bam HI + EcoRI for 8/7 and xhoI for 0/1.
- Gel Results
- Gel was unsuccessful for 1A but results for other plasmids worked well.
- Miniprep
- We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them.
- Colonies were then inoculated using LB + Amp solution and left overnight.
- Glycerol Stock
- Glycerol Stock was performed for plasmids 7/8 and 0.