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Alberta NINT/17 May 2008
From 2008.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
PCR of T/A I oligonucleotide using Term + and Term - primers | PCR of T/A I oligonucleotide using Term + and Term - primers | ||
| - | DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for insertion into plasmid. | + | DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for |
| + | insertion into plasmid. | ||
Revision as of 20:05, 9 June 2008
lab work (SD, JD):
PCR of T/A I oligonucleotide using Term + and Term - primers
DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for
insertion into plasmid.
