Team:Warsaw/Calendar-Main/10 June 2008

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(Difference between revisions)
(New page: {{WarNotebook}} <!-- do not edit above me! --> <p>1. Plasmid isolation from previous day colonies.<br> 2. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerea...)
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<p>1. Plasmid isolation from previous day colonies.<br>
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<p>1. Plasmid isolation from previous day's colonies.<br>
2. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion with HindIII and SalI<br>
2. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion with HindIII and SalI<br>
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6. DNA electrophoresis of digestion products<br>
6. DNA electrophoresis of digestion products<br>
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7. Choose proper colonies of transformants<br>
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7. Choice of proper colonies of transformants<br>

Revision as of 20:22, 14 September 2008

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1. Plasmid isolation from previous day's colonies.
2. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion with HindIII and SalI
3. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion with HindIII and EcoRI
4. Control digest of pMPM-T5 + T7 RNA-polimerase with EcoRI
5. Control digest of pMPM-T5 + T7 RNA-polimerase with BamHI and XhoI
6. DNA electrophoresis of digestion products
7. Choice of proper colonies of transformants

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