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Team:Warsaw/Calendar-Main/19 June 2008
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<p>Primers for fusion of protein A and ompA and alpha or omega part of beta-lactamase were designed:</p> | <p>Primers for fusion of protein A and ompA and alpha or omega part of beta-lactamase were designed:</p> | ||
| - | <p>alphaL+link14+homo<br> CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CACCCAGAAACGCTGGTGAAAG<br> | + | <p>alphaL+link14+homo (alphaL_link)<br> CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CACCCAGAAACGCTGGTGAAAG<br> |
| - | alphaP+STOP+XbaI+BamHI<br> GG GGATCC GG TCTAGA TTA TTCGCCAGTTAATAGTTTGCG<br> | + | alphaP+STOP+XbaI+BamHI (alphaP_XB)<br> GG GGATCC GG TCTAGA TTA TTCGCCAGTTAATAGTTTGCG<br> |
| - | omegaL+link14+homo<br> CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CTACTTACTCTAGCTTCCCGG<br> | + | omegaL+link14+homo (omegaL_link)<br> CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CTACTTACTCTAGCTTCCCGG<br> |
| - | omegaP+STOP+EcoRI+PstI+BamHI<br> AAGGATCCAACTGCAGAAGAATTC TTACCAATGCTTAATCAGTG<br> | + | omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)<br> AAGGATCCAACTGCAGAAGAATTC TTACCAATGCTTAATCAGTG<br> |
| - | OmpAL+NdeI<br> CATATGAAAGCTACTAAACTGGTACTGGG<br> | + | OmpAL+NdeI (ompaL_N)<br> CATATGAAAGCTACTAAACTGGTACTGGG<br> |
| - | OmpAP+link8+SacI+NotI+homo<br> GCCACCGCTTCCACCACT GCGGCCGC CA GAGCTC CCTCCTCCAGAACCTCC GCCGTTGTCCGGACGAGTGCCGATG</p> | + | OmpAP+link8+SacI+NotI+homo (ompaP_link)<br> GCCACCGCTTCCACCACT GCGGCCGC CA GAGCTC CCTCCTCCAGAACCTCC GCCGTTGTCCGGACGAGTGCCGATG</p> |
Revision as of 18:13, 23 September 2008
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Primers for fusion of protein A and ompA and alpha or omega part of beta-lactamase were designed: alphaL+link14+homo (alphaL_link)
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