Team:BCCS-Bristol/Notebook

From 2008.igem.org

(Difference between revisions)
(→Lab Notebook)
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Since August, we started to make a weekly summaries of our lab work progress. You find them on our [[Team:BCCS-Bristol/Notebook-Progress|Progress Page]].
Since August, we started to make a weekly summaries of our lab work progress. You find them on our [[Team:BCCS-Bristol/Notebook-Progress|Progress Page]].
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===Protocols===
===Protocols===
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* [[Team:BCCS-Bristol/Protocols-Testing_the_activity_of_biobrick_T9002|'''Testing the activity of biobrick T9002''']]
* [[Team:BCCS-Bristol/Protocols-Testing_the_activity_of_biobrick_T9002|'''Testing the activity of biobrick T9002''']]
<br>
<br>
 +
===Progress Reports===
 +
 +
* [[Team:BCCS-Bristol/Notebook-Progress#1st_July_-_8th July|'''1st July - 8th July ''']]
 +
** Swimming agar assay
 +
 +
* [[Team:BCCS-Bristol/Notebook-Progress#9th_July_-_15th July|'''9th July - 15th July ''']]
 +
 +
* [[Team:BCCS-Bristol/Notebook-Progress#16th_July_-_22nd July|'''16th July - 22nd July ''']]
 +
 +
* [[Team:BCCS-Bristol/Notebook-Progress#23rd_July_-_29th July|'''23rd July - 29th July ''']]
 +
 +
* [[Team:BCCS-Bristol/Notebook-Progress#30th_July_-_6th August|'''31st July - 6th August''']]
 +
** BioBrick Transformation
 +
*** Heat shock transformations with BioBrick DNA resulted in zero, one or two colonies per attempt
 +
*** All colonies have been verified to possess the plasmids
 +
*** Electroporation is much more successful
 +
** Agar plug assay
 +
***''E. coli'' MG1655 moved apparently some beads!!!
 +
***but unfortunenately, no chemotaxis was observed
 +
 +
* [[Team:BCCS-Bristol/Notebook-Progress#7th_August_-_13th August|'''7th August - 13th August''']]
 +
** BioBrick Transformation
 +
***The self-made electro competent ''E. coli'' DH5α cells are working
 +
***A longer incubation time of the punched paper disc in water seems to resolve more BioBrick DNA
 +
 +
* [[Team:BCCS-Bristol/Notebook-Progress#14th_August_-_20th August|'''14th August - 20th August''']]
 +
** Agar plug assay
 +
*** Repulsion achieved to some degree in the agar-plug assay.
 +
** BioBrick transformation
 +
*** DH5α transformed with pRZ1(pRS415 containing cpxR responsive promotor - sent to us by T.Silhavy, Princeton)
 +
 +
* [[Team:BCCS-Bristol/Notebook-Progress#2nd_September_-_9th September|'''2nd September - 9th September''']]
 +
** BioBrick transformation
 +
 +
* [[Team:BCCS-Bristol/Notebook-Progress#10th_September_-_16th September|'''10th September - 16th September''']]
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</div>
</div>

Revision as of 15:34, 23 September 2008

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BCCS-Notebook-Header 01.jpg BCCS-Notebook-Header 02.jpg BCCS-Notebook-Header 03.jpg


Lab Notebook

This section holds our team lab notebook which can be accessed by date. Click on any of the red dates to see what we got up to.

July
MTWTFSS
  [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/1_July_2008 1] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/2_July_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/3_July_2008&action=edit 3] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/4_July_2008 4] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/5_July_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/6_July_2008&action=edit 6]
[http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/7_July_2008 7] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/8_July_2008 8] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/9_July_2008 9] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/10_July_2008 10] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/11_July_2008 11] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/12_July_2008 12] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/13_July_2008&action=edit 13]
[http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/14_July_2008 14] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/15_July_2008 15] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/16_July_2008 16] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/17_July_2008 17] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/18_July_2008 18] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/19_July_2008 19] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/20_July_2008 20]
[http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/21_July_2008 21] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/22_July_2008 22] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/23_July_2008 23] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/24_July_2008 24] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/25_July_2008 25] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/26_July_2008 26] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/27_July_2008 27]
[http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/28_July_2008 28] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/29_July_2008 29] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/30_July_2008 30] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/1_August_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/2_August_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/3_August_2008&action=edit 3]
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/4_August_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/5_August_2008&action=edit 5] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/6_August_2008 6] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/7_August_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/8_August_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/9_August_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/10_August_2008&action=edit 10]
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/11_August_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/12_August_2008&action=edit 12] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/13_August_2008 13] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/14_August_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/15_August_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/16_August_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/17_August_2008&action=edit 17]
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/18_August_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/19_August_2008&action=edit 19] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/20_August_2008 20] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/21_August_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/22_August_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/23_August_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/24_August_2008&action=edit 24]
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/25_August_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/26_August_2008&action=edit 26] [http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/27_August_2008 27] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/28_August_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/29_August_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/30_August_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/31_August_2008&action=edit 31]
September
MTWTFSS
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/1_September_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/2_September_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/3_September_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/4_September_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/5_September_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/6_September_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/7_September_2008&action=edit 7]
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/8_September_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/9_September_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/10_September_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/11_September_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/12_September_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/13_September_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/14_September_2008&action=edit 14]
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/15_September_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/16_September_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/17_September_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/18_September_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/19_September_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/20_September_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/21_September_2008&action=edit 21]
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/22_September_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/23_September_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/24_September_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/25_September_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/26_September_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/27_September_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/28_September_2008&action=edit 28]
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/29_September_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/30_September_2008&action=edit 30]
October
MTWTFSS
    [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/1_October_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/2_October_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/3_October_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/4_October_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/5_October_2008&action=edit 5]
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/6_October_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/7_October_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/8_October_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/9_October_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/10_October_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/11_October_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/12_October_2008&action=edit 12]
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/13_October_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/14_October_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/15_October_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/16_October_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/17_October_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/18_October_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/19_October_2008&action=edit 19]
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/20_October_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/21_October_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/22_October_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/23_October_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/24_October_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/25_October_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/26_October_2008&action=edit 26]
[http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/27_October_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/28_October_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/29_October_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/30_October_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Team:BCCS-Bristol/Calendar-Notebook/31_October_2008&action=edit 31]

Since August, we started to make a weekly summaries of our lab work progress. You find them on our Progress Page.

Protocols


Progress Reports

  • 31st July - 6th August
    • BioBrick Transformation
      • Heat shock transformations with BioBrick DNA resulted in zero, one or two colonies per attempt
      • All colonies have been verified to possess the plasmids
      • Electroporation is much more successful
    • Agar plug assay
      • E. coli MG1655 moved apparently some beads!!!
      • but unfortunenately, no chemotaxis was observed
  • 7th August - 13th August
    • BioBrick Transformation
      • The self-made electro competent E. coli DH5α cells are working
      • A longer incubation time of the punched paper disc in water seems to resolve more BioBrick DNA
  • 14th August - 20th August
    • Agar plug assay
      • Repulsion achieved to some degree in the agar-plug assay.
    • BioBrick transformation
      • DH5α transformed with pRZ1(pRS415 containing cpxR responsive promotor - sent to us by T.Silhavy, Princeton)


Retrieved from "http://2008.igem.org/Team:BCCS-Bristol/Notebook"