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Virginia/22 September 2008
From 2008.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
**30min at room temp | **30min at room temp | ||
**6:1 volume ratio of melted gel of insert:vector | **6:1 volume ratio of melted gel of insert:vector | ||
| + | *Transformed cells & plated | ||
| + | **5ul DNA into 30ul competent cells | ||
[[Team:Virginia/Notebook|Back to Notebook]] | [[Team:Virginia/Notebook|Back to Notebook]] | ||
Latest revision as of 02:19, 23 September 2008
- Melted cut gel parts - ~69C for 10min
- Ligations for A73-75
- 30min at room temp
- 6:1 volume ratio of melted gel of insert:vector
- Transformed cells & plated
- 5ul DNA into 30ul competent cells
