Team:Warsaw/Calendar-Main/3 July 2008

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<h3>Preparation of constructs with OmpA protein fusions
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<br>Michał K.</h3>
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<p>'''Preparation of constructs with OmpA protein fusions'''<br>
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<li> Clean-up of overnight digestion </li>
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1. Clean-up of overnight digestation <br>
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<li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_alfa and pET15b </li>
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2. Ligation of OmpA_alfa and pET15b <br>
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<li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_omega and pET15b</li>
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3. Ligation of OmpA_omega and pET15b<br>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> TOP10 strain with ligation products </li>
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4. Transformation of E. coli TOP10 strain with these ligations <br>
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<li> Transformants plating on LB + ampicillin</li></ol>
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5. Transformants plating on LB + ampicillin
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Revision as of 16:40, 1 October 2008

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Preparation of constructs with OmpA protein fusions
Michał K.

  1. Clean-up of overnight digestion
  2. Ligation of OmpA_alfa and pET15b
  3. Ligation of OmpA_omega and pET15b
  4. Transformation of E. coli TOP10 strain with ligation products
  5. Transformants plating on LB + ampicillin

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